Regulation of glutamate uptake in astrocytes continuously exposed to ethanol - PubMed (original) (raw)
Regulation of glutamate uptake in astrocytes continuously exposed to ethanol
T L Smith. Life Sci. 1997.
Abstract
CNS glutamatergic transmission is altered by chronic ethanol intake and may underlie the behavioral hyperactivity associated with ethanol withdrawal. Because astrocytes regulate extracellular glutamate levels, the aim of this investigation was to characterize the effects of in vitro ethanol exposure on Na+-dependent glutamate uptake parameters in astrocytes. Ethanol exposure elicited a time and concentration-dependent increase in the maximal uptake capacity, Vmax, for [3H] glutamate, which was reversed upon withdrawal of ethanol from the media. None of the ethanol exposures had any effect on the Km for this process. In addition, the ethanol-induced increase in Vmax for glutamate was reversed by the protein kinase C inhibitors, calphostin C and bisindolylmaleimide, and was not associated with an increase in the expression of either of the major glutamate transporter proteins, GLT-1 or GLAST.
Similar articles
- Increased Na(+)-dependent high affinity uptake of glutamate in astrocytes chronically exposed to ethanol.
Smith TL, Zsigo A. Smith TL, et al. Neurosci Lett. 1996 Nov 1;218(2):142-4. doi: 10.1016/s0304-3940(96)13123-2. Neurosci Lett. 1996. PMID: 8945748 - Acute up-regulation of glutamate uptake mediated by mGluR5a in reactive astrocytes.
Vermeiren C, Najimi M, Vanhoutte N, Tilleux S, de Hemptinne I, Maloteaux JM, Hermans E. Vermeiren C, et al. J Neurochem. 2005 Jul;94(2):405-16. doi: 10.1111/j.1471-4159.2005.03216.x. J Neurochem. 2005. PMID: 15998291 - Increased calcium/calmodulin protein kinase activity in astrocytes chronically exposed to ethanol: influences on glutamate transport.
Smith TL, Navratilova E. Smith TL, et al. Neurosci Lett. 1999 Jul 16;269(3):145-8. doi: 10.1016/s0304-3940(99)00438-3. Neurosci Lett. 1999. PMID: 10454153 - Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.
Othman T, Sinclair CJ, Haughey N, Geiger JD, Parkinson FE. Othman T, et al. Neurochem Res. 2002 Apr;27(4):289-96. doi: 10.1023/a:1014955111742. Neurochem Res. 2002. PMID: 11958530 - Ligands of peroxisome proliferator-activated receptor-alpha promote glutamate transporter-1 endocytosis in astrocytes.
Huang HT, Liao CK, Chiu WT, Tzeng SF. Huang HT, et al. Int J Biochem Cell Biol. 2017 May;86:42-53. doi: 10.1016/j.biocel.2017.03.008. Epub 2017 Mar 18. Int J Biochem Cell Biol. 2017. PMID: 28323206
Cited by
- Alcohol drinking and deprivation alter basal extracellular glutamate concentrations and clearance in the mesolimbic system of alcohol-preferring (P) rats.
Ding ZM, Rodd ZA, Engleman EA, Bailey JA, Lahiri DK, McBride WJ. Ding ZM, et al. Addict Biol. 2013 Mar;18(2):297-306. doi: 10.1111/adb.12018. Epub 2012 Dec 14. Addict Biol. 2013. PMID: 23240885 Free PMC article. - Nifedipine Prevents Apoptosis of Alcohol-Exposed First-Trimester Trophoblast Cells.
Bolnick AD, Bolnick JM, Kohan-Ghadr HR, Kilburn BA, Hertz M, Dai J, Drewlo S, Armant DR. Bolnick AD, et al. Alcohol Clin Exp Res. 2018 Jan;42(1):53-60. doi: 10.1111/acer.13534. Epub 2017 Nov 22. Alcohol Clin Exp Res. 2018. PMID: 29048755 Free PMC article. - Ceftriaxone treatment affects the levels of GLT1 and ENT1 as well as ethanol intake in alcohol-preferring rats.
Sari Y, Sreemantula SN, Lee MR, Choi DS. Sari Y, et al. J Mol Neurosci. 2013 Nov;51(3):779-87. doi: 10.1007/s12031-013-0064-y. Epub 2013 Jul 27. J Mol Neurosci. 2013. PMID: 23893122 Free PMC article. - Astrocyte Reactivity and Neurodegeneration in the Female Rat Brain Following Alcohol Dependence.
Guerin SP, Melbourne JK, Dang HQ, Shaji CA, Nixon K. Guerin SP, et al. Neuroscience. 2023 Oct 1;529:183-199. doi: 10.1016/j.neuroscience.2023.08.016. Epub 2023 Aug 19. Neuroscience. 2023. PMID: 37598836 Free PMC article. - Ceftriaxone, a beta-lactam antibiotic, reduces ethanol consumption in alcohol-preferring rats.
Sari Y, Sakai M, Weedman JM, Rebec GV, Bell RL. Sari Y, et al. Alcohol Alcohol. 2011 May-Jun;46(3):239-46. doi: 10.1093/alcalc/agr023. Epub 2011 Mar 19. Alcohol Alcohol. 2011. PMID: 21422004 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources