CD66-mediated phagocytosis of Opa52 Neisseria gonorrhoeae requires a Src-like tyrosine kinase- and Rac1-dependent signalling pathway - PubMed (original) (raw)

CD66-mediated phagocytosis of Opa52 Neisseria gonorrhoeae requires a Src-like tyrosine kinase- and Rac1-dependent signalling pathway

C R Hauck et al. EMBO J. 1998.

Abstract

The interaction of Neisseria gonorrhoeae with human phagocytes is a hallmark of gonococcal infections. Recently, CD66 molecules have been characterized as receptors for Opa52-expressing gonococci on human neutrophils. Here we show that Opa52-expressing gonococci or Escherichia coli or F(ab) fragments directed against CD66, respectively, activate a signalling cascade from CD66 via Src-like protein tyrosine kinases, Rac1 and PAK to Jun-N-terminal kinase. The induced signal is distinct from Fcgamma-receptor-mediated signalling and is specific for Opa52, since piliated Opa- gonococci, commensal Neisseria cinerea or E.coli do not stimulate this signalling pathway. Inhibition of Src-like kinases or Rac1 prevents the uptake of Opa52 bacteria, demonstrating the crucial role of this signalling cascade for the opsonin-independent, Opa52/CD66-mediated phagocytosis of pathogenic Neisseria.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Curr Biol. 1997 Mar 1;7(3):202-10 - PubMed
    1. Infect Immun. 1997 May;65(5):1863-9 - PubMed
    1. EMBO J. 1997 Jun 16;16(12):3435-45 - PubMed
    1. Infect Immun. 1997 Oct;65(10):4122-9 - PubMed
    1. Infect Immun. 1975 Jun;11(6):1352-61 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources