Sex-specific exons control DNA methyltransferase in mammalian germ cells - PubMed (original) (raw)
Comparative Study
. 1998 Mar;125(5):889-97.
doi: 10.1242/dev.125.5.889.
Affiliations
- PMID: 9449671
- DOI: 10.1242/dev.125.5.889
Comparative Study
Sex-specific exons control DNA methyltransferase in mammalian germ cells
C Mertineit et al. Development. 1998 Mar.
Abstract
The spermatozoon and oocyte genomes bear sex-specific methylation patterns that are established during gametogenesis and are required for the allele-specific expression of imprinted genes in somatic tissues. The mRNA for Dnmt1, the predominant maintenance and de novo DNA (cytosine-5)-methyl transferase in mammals, is present at high levels in postmitotic murine germ cells but undergoes alternative splicing of sex-specific 5' exons, which controls the production and localization of enzyme during specific stages of gametogenesis. An oocyte-specific 5' exon is associated with the production of very large amounts of active Dnmt1 protein, which is truncated at the N terminus and sequestered in the cytoplasm during the later stages of oocyte growth, while a spermatocyte-specific 5' exon interferes with translation and prevents production of Dnmt1 during the prolonged crossing-over stage of male meiosis. During the course of postnatal oogenesis, Dnmt1 is present at high levels in nuclei only in growing dictyate oocytes, a stage during which gynogenetic developmental potential is lost and biparental developmental potential is gained.
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