New protein kinase and protein phosphatase families mediate signal transduction in bacterial catabolite repression - PubMed (original) (raw)

Multiple alignment of HPr kinase sequences. A consensus sequence was indicated when, for a given position, an identical amino acid was found in at least five HPr kinase sequences. A sequence corresponding to the putative A motif or P-loop (24) of nucleotide binding proteins (GXXXXGK[TS]) is located in positions 153–160 of the B. subtilis HPr kinase. The different sequences can be found on the World Wide Web at the following Internet addresses: E. faecalis (

http://expasy.hcuge.ch/sprot/sprot\_top.html

, Swiss-Prot accession no. 007664); S. pyogenes (

http://www.ncbi.nlm.nih.gov/cgi-bin/BLAST/nph-ouacgtbl

(contig183)); M. pneumoniae (

http://nbrfa.georgetown.edu/nbrf/get.html

, Protein Identification Resource, accession no. S73934); M. genitalium (

http://expasy.hcuge.ch/sprot/sprot\_top.html

, Swiss-Prot accession no. P47331); T. pallidum [

http://www.ncbi.nlm.nih.gov/cgi-bin/BLAST/nph-tigrbl

(tp_5580)]; N. gonorrhoeae [

http://www.ncbi.nlm.nih.gov/cgi-bin/BLAST/nph-ouacgtbl

(contig268)]; N. meningitidis (

http://www.ncbi.nlm.nih.gov/cgi-bin/BLAST/nph-tigrbl

); and C. acetobutylicum (

http://www.genomecorp.com/htdocs/sequences/clostridium/clospage.html

). The genome sequences of E. faecalis, S. pyogenes, N. gonorrhoeae, N. meningitidis, and C. acetobutylicum are not yet completed. The hprK sequences of E. faecalis, S. pyogenes, and N. meningitidis are incomplete, and semicolons in the deduced HPr kinase sequences represent unknown amino acids. We used the taxonomy browser from the National Center for Biotechnology Information to characterize the position of each bacterium.