Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions - PubMed (original) (raw)
Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions
T E Barrett et al. Cell. 1998.
Free article
Abstract
G:U mismatches resulting from deamination of cytosine are the most common promutagenic lesions occurring in DNA. Uracil is removed in a base-excision repair pathway by uracil DNA-glycosylase (UDG), which excises uracil from both single- and double-stranded DNA. Recently, a biochemically distinct family of DNA repair enzymes has been identified, which excises both uracil and thymine, but only from mispairs with guanine. Crystal structures of the mismatch-specific uracil DNA-glycosylase (MUG) from E. coli, and of a DNA complex, reveal a remarkable structural and functional homology to UDGs despite low sequence identity. Details of the MUG structure explain its thymine DNA-glycosylase activity and the specificity for G:U/T mispairs, which derives from direct recognition of guanine on the complementary strand.
Similar articles
- A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase.
Gallinari P, Jiricny J. Gallinari P, et al. Nature. 1996 Oct 24;383(6602):735-8. doi: 10.1038/383735a0. Nature. 1996. PMID: 8878487 - Crystal structure of a thwarted mismatch glycosylase DNA repair complex.
Barrett TE, Schärer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH. Barrett TE, et al. EMBO J. 1999 Dec 1;18(23):6599-609. doi: 10.1093/emboj/18.23.6599. EMBO J. 1999. PMID: 10581234 Free PMC article. - Structure and function in the uracil-DNA glycosylase superfamily.
Pearl LH. Pearl LH. Mutat Res. 2000 Aug 30;460(3-4):165-81. doi: 10.1016/s0921-8777(00)00025-2. Mutat Res. 2000. PMID: 10946227 Review. - 3,N4-ethenocytosine, a highly mutagenic adduct, is a primary substrate for Escherichia coli double-stranded uracil-DNA glycosylase and human mismatch-specific thymine-DNA glycosylase.
Saparbaev M, Laval J. Saparbaev M, et al. Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8508-13. doi: 10.1073/pnas.95.15.8508. Proc Natl Acad Sci U S A. 1998. PMID: 9671708 Free PMC article. - Uracil in DNA--occurrence, consequences and repair.
Krokan HE, Drabløs F, Slupphaug G. Krokan HE, et al. Oncogene. 2002 Dec 16;21(58):8935-48. doi: 10.1038/sj.onc.1205996. Oncogene. 2002. PMID: 12483510 Review.
Cited by
- Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation Sequencing.
Baljinnyam T, Conrad JW, Sowers ML, Chang-Gu B, Herring JL, Hackfeld LC, Zhang K, Sowers LC. Baljinnyam T, et al. Chem Res Toxicol. 2023 Feb 20;36(2):162-176. doi: 10.1021/acs.chemrestox.2c00172. Epub 2023 Jan 16. Chem Res Toxicol. 2023. PMID: 36647573 Free PMC article. - Interaction landscape of a 'CαNN' motif with arsenate and arsenite: a potential peptide-based scavenger of arsenic.
Sahu S, Sheet T, Banerjee R. Sahu S, et al. RSC Adv. 2019 Jan 9;9(2):1062-1074. doi: 10.1039/c8ra08225a. eCollection 2019 Jan 2. RSC Adv. 2019. PMID: 35517606 Free PMC article. - The influence of base pair tautomerism on single point mutations in aqueous DNA.
Gheorghiu A, Coveney PV, Arabi AA. Gheorghiu A, et al. Interface Focus. 2020 Dec 6;10(6):20190120. doi: 10.1098/rsfs.2019.0120. Epub 2020 Oct 16. Interface Focus. 2020. PMID: 33178413 Free PMC article. - Horizontal Gene Transfer to a Defensive Symbiont with a Reduced Genome in a Multipartite Beetle Microbiome.
Waterworth SC, Flórez LV, Rees ER, Hertweck C, Kaltenpoth M, Kwan JC. Waterworth SC, et al. mBio. 2020 Feb 25;11(1):e02430-19. doi: 10.1128/mBio.02430-19. mBio. 2020. PMID: 32098813 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases