Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover - PubMed (original) (raw)

. 1998 Mar 1;160(5):2166-73.

Affiliations

PMID: 9498754

Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover

P J Leenen et al. J Immunol. 1998.

Abstract

In the normal mouse spleen, two distinct populations of dendritic cells (DC) are present that differ in microanatomical location. The major population of marginal DC is found in the "marginal zone bridging channels" and extends into the red pulp. The interdigitating cells (IDC) are localized in the T cell areas in the white pulp. The aim of the present study was to characterize these two splenic DC populations with regard to their phenotype, in vivo phagocytic function, and turnover. Both marginal DC and IDC are CD11c+ and CD13+, but only IDC are NLDC-145+ and CD8alpha+. Notably, both populations, when freshly isolated, express the macrophage markers F4/80, BM8, and Mac-1. To study the phagocytic capacity of these cells, we employed the macrophage "suicide" technique by injecting liposomes loaded with clodronate i.v. Marginal DC, but not IDC, were eliminated by this treatment. Phagocytosis of DiI-labeled liposomes by DC confirmed this finding. The two DC populations differed significantly with regard to their turnover rates, as studied in a transgenic mouse model of conditional depletion of DC populations with high turnover. In these mice, marginal DC were completely eliminated, but the IDC population remained virtually intact. From these data we conclude that the marginal DC population has a high turnover, in contrast to the IDC population. Taken together, the present results indicate that marginal DC and IDC represent two essentially distinct populations of DC in the mouse spleen. They differ not only in location, but also in phenotype, phagocytic ability, and turnover.

PubMed Disclaimer

Cited by

Chess Not Checkers: Complexities Within the Myeloid Response to the Acute Kidney Injury Syndrome.
Nash WT, Okusa MD. Nash WT, et al. Front Med (Lausanne). 2021 May 26;8:676688. doi: 10.3389/fmed.2021.676688. eCollection 2021. Front Med (Lausanne). 2021. PMID: 34124107 Free PMC article. Review.
Patrolling monocytes inhibit osteosarcoma metastasis to the lung.
Chen T, Zhao L. Chen T, et al. Aging (Albany NY). 2020 Nov 16;12(22):23004-23016. doi: 10.18632/aging.104041. Epub 2020 Nov 16. Aging (Albany NY). 2020. PMID: 33203789 Free PMC article.
Compartmentalization of dendritic cell and T-cell interactions in the lymph node: Anatomy of T-cell fate decisions.
León B, Lund FE. León B, et al. Immunol Rev. 2019 May;289(1):84-100. doi: 10.1111/imr.12758. Immunol Rev. 2019. PMID: 30977197 Free PMC article. Review.
Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution.
Weinstock A, Fisher EA. Weinstock A, et al. Methods Mol Biol. 2019;1951:153-165. doi: 10.1007/978-1-4939-9130-3_12. Methods Mol Biol. 2019. PMID: 30825151 Free PMC article.
The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation.
Nechama M, Kwon J, Wei S, Kyi AT, Welner RS, Ben-Dov IZ, Arredouani MS, Asara JM, Chen CH, Tsai CY, Nelson KF, Kobayashi KS, Israel E, Zhou XZ, Nicholson LK, Lu KP. Nechama M, et al. Nat Commun. 2018 Apr 23;9(1):1603. doi: 10.1038/s41467-018-03886-6. Nat Commun. 2018. PMID: 29686383 Free PMC article. Clinical Trial.

Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover - PubMed (original) (raw)