Translational control: a general mechanism for gene regulation during T cell activation - PubMed (original) (raw)
Translational control: a general mechanism for gene regulation during T cell activation
J A Garcia-Sanz et al. FASEB J. 1998 Mar.
Abstract
Distributional changes of individual mRNAs between free ribonucleoprotein particles (mRNP) and ribosome-bound transcripts are used to assess translational control. Simultaneous analysis of many mRNA species is required to estimate the overall contribution of translation to the regulation of gene expression. To this purpose, total cytoplasmic RNA was fractionated in sucrose step gradients and poly(A)+ RNA was prepared from mRNP and ribosome-bound fractions. Since direct, simultaneous analysis of a profusion of mRNAs is not feasible, distribution of their in vitro translation products was examined after separation in 2-dimensional gels, followed by computer-based analysis of autoradiographs. When this analysis was applied to antigenically stimulated T cells, 36% of in vitro translation products showed a greater than 10-fold increase in intensity, suggesting transcriptional activation of the corresponding mRNAs. In comparison, 7.9% of individual mRNAs (54 of 685 species) were translationally activated. They were redistributed from free mRNP to ribosome-associated fractions; 4.7% (32 species) were translationally repressed, as indicated by the opposite pattern. The differential recruitment of 12.6% of mRNA species demonstrates specificity and the general significance of translational control during T cell activation, which implies that translation may play a similar role in regulating gene expression in a variety of physiological processes.
Similar articles
- Isolation of translationally controlled mRNAs by differential screening.
Mikulits W, Pradet-Balade B, Habermann B, Beug H, Garcia-Sanz JA, Müllner EW. Mikulits W, et al. FASEB J. 2000 Aug;14(11):1641-52. doi: 10.1096/fj.14.11.1641. FASEB J. 2000. PMID: 10928999 - Detection of early gene expression changes during activation of human primary lymphocytes by in vitro synthesis of proteins from polysome-associated mRNAs.
Miyamoto S, Qin J, Safer B. Miyamoto S, et al. Protein Sci. 2001 Feb;10(2):423-33. doi: 10.1110/ps.21301. Protein Sci. 2001. PMID: 11266628 Free PMC article. - Proteomic characterization of messenger ribonucleoprotein complexes bound to nontranslated or translated poly(A) mRNAs in the rat cerebral cortex.
Angenstein F, Evans AM, Ling SC, Settlage RE, Ficarro S, Carrero-Martinez FA, Shabanowitz J, Hunt DF, Greenough WT. Angenstein F, et al. J Biol Chem. 2005 Feb 25;280(8):6496-503. doi: 10.1074/jbc.M412742200. Epub 2004 Dec 13. J Biol Chem. 2005. PMID: 15596439 - Patterns, mechanisms, and functions of translation regulation in mammalian spermatogenic cells.
Kleene KC. Kleene KC. Cytogenet Genome Res. 2003;103(3-4):217-24. doi: 10.1159/000076807. Cytogenet Genome Res. 2003. PMID: 15051942 Review. - Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation.
Weston A, Sommerville J. Weston A, et al. Nucleic Acids Res. 2006 Jun 12;34(10):3082-94. doi: 10.1093/nar/gkl409. Print 2006. Nucleic Acids Res. 2006. PMID: 16769775 Free PMC article. Review.
Cited by
- mTOR-dependent translation drives tumor infiltrating CD8+ effector and CD4+ Treg cells expansion.
De Ponte Conti B, Miluzio A, Grassi F, Abrignani S, Biffo S, Ricciardi S. De Ponte Conti B, et al. Elife. 2021 Nov 17;10:e69015. doi: 10.7554/eLife.69015. Elife. 2021. PMID: 34787568 Free PMC article. - Mechanistic Target of Rapamycin Complex 1 (mTORC1) and mTORC2 as Key Signaling Intermediates in Mesenchymal Cell Activation.
Walker NM, Belloli EA, Stuckey L, Chan KM, Lin J, Lynch W, Chang A, Mazzoni SM, Fingar DC, Lama VN. Walker NM, et al. J Biol Chem. 2016 Mar 18;291(12):6262-71. doi: 10.1074/jbc.M115.672170. Epub 2016 Jan 11. J Biol Chem. 2016. PMID: 26755732 Free PMC article. - Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells.
Buettner F, Natarajan KN, Casale FP, Proserpio V, Scialdone A, Theis FJ, Teichmann SA, Marioni JC, Stegle O. Buettner F, et al. Nat Biotechnol. 2015 Feb;33(2):155-60. doi: 10.1038/nbt.3102. Epub 2015 Jan 19. Nat Biotechnol. 2015. PMID: 25599176 - Distinct translational control in CD4+ T cell subsets.
Bjur E, Larsson O, Yurchenko E, Zheng L, Gandin V, Topisirovic I, Li S, Wagner CR, Sonenberg N, Piccirillo CA. Bjur E, et al. PLoS Genet. 2013 May;9(5):e1003494. doi: 10.1371/journal.pgen.1003494. Epub 2013 May 2. PLoS Genet. 2013. PMID: 23658533 Free PMC article. - Mammalian genes preferentially co-retained in radiation hybrid panels tend to avoid coexpression.
Liao BY, Chang AY. Liao BY, et al. PLoS One. 2012;7(2):e32284. doi: 10.1371/journal.pone.0032284. Epub 2012 Feb 24. PLoS One. 2012. PMID: 22384204 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources