Proliferation-dependent replication in primary macrophages of macrophage-tropic HIV type 1 variants - PubMed (original) (raw)
Proliferation-dependent replication in primary macrophages of macrophage-tropic HIV type 1 variants
N A Kootstra et al. AIDS Res Hum Retroviruses. 1998.
Abstract
We previously demonstrated that completion of reverse transcription in macrophages inoculated with the HIV-1 Ba-L variant was established only in the subpopulation of cells with proliferative capacity. In our present study we further extended this observation with three additional HIV-1 isolates, being the macrophage-tropic ADA strain and two primary macrophage-tropic HIV-1 variants isolated from cerebrospinal fluid and from bronchoalveolar lavage from AIDS patients. On inoculation, irrespective of the virus variant used, elongated reverse transcription products could be demonstrated only in macrophages that had proliferated during inoculation as evidenced by the incorporation of bromodeoxyuridine (BrdU), a thymidine analog. The presence of newly synthesized early products of reverse transcription also in the BrdU-negative fraction indicated that viral entry is not disturbed in nondividing cells. Our data indicate that the process of reverse transcription is dependent on cellular conditions that coincide with cell proliferation, and therefore that HIV-1 replication is restricted to cells with proliferative potential.
Similar articles
- HIV-1 macrophage tropism is determined at multiple levels of the viral replication cycle.
Fouchier RA, Brouwer M, Kootstra NA, Huisman HG, Schuitemaker H. Fouchier RA, et al. J Clin Invest. 1994 Nov;94(5):1806-14. doi: 10.1172/JCI117529. J Clin Invest. 1994. PMID: 7962525 Free PMC article. - Suppression of HIV replication in human monocyte-derived macrophages induced by granulocyte/macrophage colony-stimulating factor.
Matsuda S, Akagawa K, Honda M, Yokota Y, Takebe Y, Takemori T. Matsuda S, et al. AIDS Res Hum Retroviruses. 1995 Sep;11(9):1031-8. doi: 10.1089/aid.1995.11.1031. AIDS Res Hum Retroviruses. 1995. PMID: 8554900 - HIV-1 entry and reverse transcription in macrophages.
O'Brien WA. O'Brien WA. J Leukoc Biol. 1994 Sep;56(3):273-7. doi: 10.1002/jlb.56.3.273. J Leukoc Biol. 1994. PMID: 8083599 Review. - Macrophage-tropic HIV-1 variants: initiators of infection and AIDS pathogenesis?
Schuitemaker H. Schuitemaker H. J Leukoc Biol. 1994 Sep;56(3):218-24. doi: 10.1002/jlb.56.3.218. J Leukoc Biol. 1994. PMID: 8083592 Review.
Cited by
- The Role of p53 in HIV Infection.
Yaseen MM, Abuharfeil NM, Darmani H. Yaseen MM, et al. Curr HIV/AIDS Rep. 2023 Dec;20(6):419-427. doi: 10.1007/s11904-023-00684-8. Epub 2023 Nov 27. Curr HIV/AIDS Rep. 2023. PMID: 38010468 Review. - Inhibition of HIV early replication by the p53 and its downstream gene p21.
Shi B, Sharifi HJ, DiGrigoli S, Kinnetz M, Mellon K, Hu W, de Noronha CMC. Shi B, et al. Virol J. 2018 Mar 27;15(1):53. doi: 10.1186/s12985-018-0959-x. Virol J. 2018. PMID: 29587790 Free PMC article. - Host hindrance to HIV-1 replication in monocytes and macrophages.
Bergamaschi A, Pancino G. Bergamaschi A, et al. Retrovirology. 2010 Apr 7;7:31. doi: 10.1186/1742-4690-7-31. Retrovirology. 2010. PMID: 20374633 Free PMC article. Review. - HIV-1 activates macrophages independent of Toll-like receptors.
Brown JN, Kohler JJ, Coberley CR, Sleasman JW, Goodenow MM. Brown JN, et al. PLoS One. 2008;3(12):e3664. doi: 10.1371/journal.pone.0003664. Epub 2008 Dec 2. PLoS One. 2008. PMID: 19048100 Free PMC article. - Human immunodeficiency virus infection inhibits granulocyte-macrophage colony-stimulating factor-induced microglial proliferation.
Cosenza-Nashat M, Zhao ML, Marshall HD, Si Q, Morgello S, Lee SC. Cosenza-Nashat M, et al. J Neurovirol. 2007 Dec;13(6):536-48. doi: 10.1080/13550280701549417. J Neurovirol. 2007. PMID: 18097885
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials