The use of spectrophotometry to estimate melanin density in Caucasians - PubMed (original) (raw)
Affiliations
- PMID: 9521433
The use of spectrophotometry to estimate melanin density in Caucasians
T Dwyer et al. Cancer Epidemiol Biomarkers Prev. 1998 Mar.
Abstract
The density of cutaneous melanin may be the property of the skin that protects it from damage by solar radiation, but there is not an accepted, noninvasive method of measuring it. To determine whether the density of cutaneous melanin can be estimated from reflectance of visible light by the skin, reflectance of 15-nm wavebands of light by the skin of the inner upper arm of each of 82 volunteers was measured at 20-nm intervals with a Minolta 508 spectrophotometer. A 3-mm skin biopsy was then taken from the same site, and four nonserial sections of it were stained with Masson Fontana for melanin. The melanin content of the basal area was calculated using the NIH Image analysis system. We show that cutaneous melanin in Caucasians can be estimated by the difference between two measurements of reflectance of visible light by the skin: those at wavelengths 400 and 420 nm. This new spectrophotometric measurement was more highly correlated (r = 0.68) with the histological measurements of cutaneous melanin than was skin reflectance of light of wavelength 680 nm (r = 0.33). Reflectances in the range of 650-700 nm have been used previously in skin cancer research. This relatively accurate measurement of melanin is quick and noninvasive and can be readily used in the field. It should provide improved discrimination of individual susceptibility to epidermal tumors in Caucasians and information about melanin's biological role in the causation of skin cancer.
Similar articles
- Cutaneous melanin density of Caucasians measured by spectrophotometry and risk of malignant melanoma, basal cell carcinoma, and squamous cell carcinoma of the skin.
Dwyer T, Blizzard L, Ashbolt R, Plumb J, Berwick M, Stankovich JM. Dwyer T, et al. Am J Epidemiol. 2002 Apr 1;155(7):614-21. doi: 10.1093/aje/155.7.614. Am J Epidemiol. 2002. PMID: 11914188 - Melanin density and melanin type predict melanocytic naevi in 19-20 year olds of northern European ancestry.
Dwyer T, Prota G, Blizzard L, Ashbolt R, Vincensi MR. Dwyer T, et al. Melanoma Res. 2000 Aug;10(4):387-94. doi: 10.1097/00008390-200008000-00011. Melanoma Res. 2000. PMID: 10985674 - Misclassification due to body hair and seasonal variation on melanin density estimates for skin type using spectrophotometry.
van der Mei IA, Blizzard L, Stankovich J, Ponsonby AL, Dwyer T. van der Mei IA, et al. J Photochem Photobiol B. 2002 Aug;68(1):45-52. doi: 10.1016/s1011-1344(02)00331-7. J Photochem Photobiol B. 2002. PMID: 12208036 - Melanin fate in the human epidermis: a reassessment of how best to detect and analyse histologically.
Joly-Tonetti N, Wibawa JI, Bell M, Tobin D. Joly-Tonetti N, et al. Exp Dermatol. 2016 Jul;25(7):501-4. doi: 10.1111/exd.13016. Exp Dermatol. 2016. PMID: 26998907 Review. - From Melanocytes to Melanoma Cells: Characterization of the Malignant Transformation by Four Distinctly Different Melanin Fluorescence Spectra (Review).
Leupold D, Pfeifer L, Hofmann M, Forschner A, Wessler G, Haenssle H. Leupold D, et al. Int J Mol Sci. 2021 May 17;22(10):5265. doi: 10.3390/ijms22105265. Int J Mol Sci. 2021. PMID: 34067690 Free PMC article. Review.
Cited by
- Results from a first-in-human study of dersimelagon, an investigational oral selective MC1R agonist.
Ogasawara A, Ogawa K, Ide R, Ikenaga Y, Fukunaga C, Nakayama S, Tsuda M. Ogasawara A, et al. Eur J Clin Pharmacol. 2023 Jun;79(6):801-813. doi: 10.1007/s00228-023-03476-6. Epub 2023 Apr 15. Eur J Clin Pharmacol. 2023. PMID: 37060458 Free PMC article. Clinical Trial. - Cumulative UV Exposure or a Modified SCINEXA™-Skin Aging Score Do Not Play a Substantial Role in Predicting the Risk of Developing Keratinocyte Cancers after Solid Organ Transplantation-A Case Control Study.
Borik-Heil L, Endler G, Parson W, Zuckermann A, Schnaller L, Uyanik-Ünal K, Jaksch P, Böhmig G, Cejka D, Staufer K, Hielle-Wittmann E, Rasoul-Rockenschaub S, Wolf P, Sunder-Plassmann R, Geusau A. Borik-Heil L, et al. Cancers (Basel). 2023 Jan 30;15(3):864. doi: 10.3390/cancers15030864. Cancers (Basel). 2023. PMID: 36765822 Free PMC article. - In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient.
Quiles J, Cabrera M, Jones J, Tsapekos M, Caturla N. Quiles J, et al. Molecules. 2022 Nov 21;27(22):8101. doi: 10.3390/molecules27228101. Molecules. 2022. PMID: 36432202 Free PMC article. - Criterion-Related Validity of Spectroscopy-Based Skin Carotenoid Measurements as a Proxy for Fruit and Vegetable Intake: A Systematic Review.
Radtke MD, Pitts SJ, Jahns L, Firnhaber GC, Loofbourrow BM, Zeng A, Scherr RE. Radtke MD, et al. Adv Nutr. 2020 Sep 1;11(5):1282-1299. doi: 10.1093/advances/nmaa054. Adv Nutr. 2020. PMID: 32407509 Free PMC article. - Multicolour imaging in isolated foveal hypoplasia.
Venkatesh R, Jain K, Yadav NK. Venkatesh R, et al. Indian J Ophthalmol. 2020 Jan;68(1):191-192. doi: 10.4103/ijo.IJO_1017_19. Indian J Ophthalmol. 2020. PMID: 31856510 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources