FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences - PubMed (original) (raw)
FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences
R B Todd et al. EMBO J. 1998.
Abstract
The facB gene is required for acetate induction of acetamidase (amdS) and the acetate utilization enzymes acetyl-CoA synthase (facA), isocitrate lyase (acuD) and malate synthase (acuE) in Aspergillus nidulans. The facB gene encodes a transcriptional activator with a GAL4-type Zn(II)2Cys6 zinc binuclear cluster DNA-binding domain which is shown to be required for DNA binding. In vitro DNA-binding sites for FacB in the 5' regions of the amdS, facA, acuD and acuE genes have been identified. Mutations in amdS FacB DNA-binding sites affected expression of an amdS-lacZ reporter in vivo and altered the affinity of in vitro DNA binding. This study shows that the FacB Zn(II)2Cys6 cluster binds to dissimilar sites which show similarity in form but not sequence with DNA-binding sites of other Zn(II)2Cys6 proteins. Sequences with homology to FacB sites are found in the 5' regions of genes regulated by the closely related yeast Zn(II)2Cys6 protein CAT8.
Similar articles
- Molecular characterization of mutants of the acetate regulatory gene facB of Aspergillus nidulans.
Todd RB, Kelly JM, Davis MA, Hynes MJ. Todd RB, et al. Fungal Genet Biol. 1997 Oct;22(2):92-102. doi: 10.1006/fgbi.1997.1007. Fungal Genet Biol. 1997. PMID: 9367656 - The amdA regulatory gene of Aspergillus nidulans: characterization of gain-of-function mutations and identification of binding sites for the gene product.
Andrianopoulos A, Brons J, Davis MA, Hynes MJ. Andrianopoulos A, et al. Fungal Genet Biol. 1997 Feb;21(1):50-63. doi: 10.1006/fgbi.1997.0968. Fungal Genet Biol. 1997. PMID: 9126617 - The acetate regulatory gene facB of Aspergillus nidulans encodes a Zn(II)2Cys6 transcriptional activator.
Todd RB, Murphy RL, Martin HM, Sharp JA, Davis MA, Katz ME, Hynes MJ. Todd RB, et al. Mol Gen Genet. 1997 May 20;254(5):495-504. doi: 10.1007/s004380050444. Mol Gen Genet. 1997. PMID: 9197408 - Evolution of a fungal regulatory gene family: the Zn(II)2Cys6 binuclear cluster DNA binding motif.
Todd RB, Andrianopoulos A. Todd RB, et al. Fungal Genet Biol. 1997 Jun;21(3):388-405. doi: 10.1006/fgbi.1997.0993. Fungal Genet Biol. 1997. PMID: 9290251 Review. - Fungal catabolic gene regulation: molecular genetic analysis of the amdS gene of Aspergillus nidulans.
Davis MA, Kelly JM, Hynes MJ. Davis MA, et al. Genetica. 1993;90(2-3):133-45. doi: 10.1007/BF01435035. Genetica. 1993. PMID: 8119589 Review.
Cited by
- The Transcription Factors AcuK and AcuM Influence Siderophore Biosynthesis of Aspergillus fumigatus.
Caballero P, Yap A, Bromley MJ, Haas H. Caballero P, et al. J Fungi (Basel). 2024 Apr 30;10(5):327. doi: 10.3390/jof10050327. J Fungi (Basel). 2024. PMID: 38786682 Free PMC article. - Aspergillus fumigatus Acetate Utilization Impacts Virulence Traits and Pathogenicity.
Ries LNA, Alves de Castro P, Pereira Silva L, Valero C, Dos Reis TF, Saborano R, Duarte IF, Persinoti GF, Steenwyk JL, Rokas A, Almeida F, Costa JH, Fill T, Sze Wah Wong S, Aimanianda V, Rodrigues FJS, Gonçales RA, Duarte-Oliveira C, Carvalho A, Goldman GH. Ries LNA, et al. mBio. 2021 Aug 31;12(4):e0168221. doi: 10.1128/mBio.01682-21. Epub 2021 Jul 27. mBio. 2021. PMID: 34311583 Free PMC article. - Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii.
Barbay D, Mačáková M, Sützl L, De S, Mattanovich D, Gasser B. Barbay D, et al. Curr Genet. 2021 Aug;67(4):641-661. doi: 10.1007/s00294-021-01165-4. Epub 2021 Mar 16. Curr Genet. 2021. PMID: 33725138 Free PMC article. - Nutritional Heterogeneity Among Aspergillus fumigatus Strains Has Consequences for Virulence in a Strain- and Host-Dependent Manner.
Ries LNA, Steenwyk JL, de Castro PA, de Lima PBA, Almeida F, de Assis LJ, Manfiolli AO, Takahashi-Nakaguchi A, Kusuya Y, Hagiwara D, Takahashi H, Wang X, Obar JJ, Rokas A, Goldman GH. Ries LNA, et al. Front Microbiol. 2019 Apr 24;10:854. doi: 10.3389/fmicb.2019.00854. eCollection 2019. Front Microbiol. 2019. PMID: 31105662 Free PMC article. - Hybrid Transcription Factor Engineering Activates the Silent Secondary Metabolite Gene Cluster for (+)-Asperlin in Aspergillus nidulans.
Grau MF, Entwistle R, Chiang YM, Ahuja M, Oakley CE, Akashi T, Wang CCC, Todd RB, Oakley BR. Grau MF, et al. ACS Chem Biol. 2018 Nov 16;13(11):3193-3205. doi: 10.1021/acschembio.8b00679. Epub 2018 Oct 29. ACS Chem Biol. 2018. PMID: 30339758 Free PMC article.
References
- J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
- Mol Cell Biol. 1983 Aug;3(8):1430-9 - PubMed
- Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 - PubMed
- Cell. 1987 Apr 10;49(1):19-27 - PubMed
- Mol Cell Biol. 1987 Mar;7(3):1256-66 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous