Induction of mucosal B-cell memory by intramuscular inoculation of mice with rotavirus - PubMed (original) (raw)

Induction of mucosal B-cell memory by intramuscular inoculation of mice with rotavirus

S E Coffin et al. J Virol. 1998 Apr.

Abstract

We investigated the capacity of intramuscular (i.m.) immunization with heterologous-host rotavirus (simian strain RRV) to induce mucosal virus-specific memory B cells in mice. We found that prior i.m. immunization enhanced the magnitude of mucosal virus-specific immunoglobulin A (IgA) production but did not alter the site and timing of induction of virus-specific IgA responses after challenge.

PubMed Disclaimer

Figures

FIG. 1

FIG. 1

Kinetics of virus-specific IgA production by PP (A), MLN (B), and LP (C) from i.m.-immunized and unimmunized animals after oral challenge. Adult BALB/c mice were inoculated i.m. with simian rotavirus strain RRV (i.m. primed). Six weeks after primary i.m. inoculation, naïve (unprimed) and i.m.-primed mice were inoculated orally with EDIM. Lymphoid cultures of systemic and gut-associated tissues were performed 0, 2, 4, 6, and 8 days after oral inoculation. Supernatant fluids were tested for the presence of rotavirus-specific and total IgA by ELISA. Virus-specific antibodies were not detected by ELISA at concentrations of <2 ng/ml. Ratios are rotavirus-specific IgA/total IgA (in nanograms per ml). ND, not done.

FIG. 2

FIG. 2

Kinetics of virus-specific IgG production by PP (A), MLN (B), and LP (C) from i.m.-immunized and unimmunized animals after oral challenge. Adult BALB/c mice were inoculated i.m. with simian rotavirus strain RRV (i.m. primed). Six weeks after primary i.m. inoculation, naïve (unprimed) and i.m.-primed mice were inoculated orally with EDIM. Lymphoid cultures of systemic and gut-associated tissues were performed 0, 2, 4, 6, and 8 days after oral inoculation. Supernatant fluids were tested for the presence of rotavirus-specific and total IgG by ELISA. Virus-specific IgG was not detected by ELISA at concentrations of <2 ng/ml. Ratios are rotavirus-specific IgG/total IgG (in nanograms per ml). ND, not done.

References

    1. Berlin C, Bargatze R F, Campbell J J, von Andrian U H, Szabo M C, Hasslen S R, Nelson R D, Berg E L, Erlandsen S L, Butcher E C. Alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell. 1995;80:413–422. -PubMed
    1. Brown K A, Moser C A, Speaker T J, Khoury C A, Offit P A. Enhancement by microencapsulation of rotavirus-specific intestinal immune responses in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture. J Infect Dis. 1995;171:1334–1338. -PubMed
    1. Buscho R F, Perkins J C, Knopf H L S, Kapikian A Z, Chanock R M. Further characterization of the local respiratory tract antibody response induced by intranasal instillation of the inactivated rhinovirus 13 vaccine. J Immunol. 1972;108:169–177. -PubMed
    1. Cebra J J, Cuff C F, Rubin D H. Relationship between alpha/beta T cell receptor/CD8+ precursors for cytotoxic T lymphocytes in the murine Peyer’s patches and the intraepithelial compartment probed by oral infection with reovirus. Immunol Res. 1991;10:321–323. -PubMed
    1. Coffin S E, Moser C A, Cohen S, Clark H F, Offit P A. Immunologic correlates of protection against rotavirus challenge after intramuscular immunization of mice. J Virol. 1997;71:7851–7856. -PMC -PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources