Modalities of distortion of physiological voltage signals by patch-clamp amplifiers: a modeling study - PubMed (original) (raw)
Modalities of distortion of physiological voltage signals by patch-clamp amplifiers: a modeling study
J Magistretti et al. Biophys J. 1998 Feb.
Abstract
An extensive evaluation of the possible alterations affecting physiological voltage signals recorded with patch-clamp amplifiers (PCAs) working in the current-clamp (CC) mode was carried out by following a modeling approach. The PCA output voltage and current signals obtained during CC recordings performed under simplified experimental conditions were exploited to determine the equations describing the generation of error currents and voltage distortions by PCAs. The functions thus obtained were used to construct models of PCAs working in the CC mode, which were coupled to numerical simulations of neuronal bioelectrical behavior; this allowed us to evaluate the effects of the same PCAs on different physiological membrane-voltage events. The models revealed that rapid signals such as fast action potentials are preferentially affected, whereas slower events, such as low-threshold spikes, are less altered. Prominent effects of model PCAs on fast action potentials were alterations of their amplitude, duration, depolarization and repolarization speeds, and, most notably, the generation of spurious afterhyperpolarizations. Processes like regular firing and burst firing could also be altered, under particular conditions, by the model PCAs. When a cell consisting of more than one single intracellular compartment was considered, the model PCAs distorted fast equalization transients. Furthermore, the effects of different experimental and cellular parameters (series resistance, cell capacitance, temperature) on PCA-generated artifacts were analyzed. Finally, the simulations indicated that no off-line correction based on manipulations of the error-current signals returned by the PCAs can be successfully performed in the attempt to recover unperturbed voltage signals, because of alterations of the overall current flowing through the cell-PCA system.
Similar articles
- Action potentials recorded with patch-clamp amplifiers: are they genuine?
Magistretti J, Mantegazza M, Guatteo E, Wanke E. Magistretti J, et al. Trends Neurosci. 1996 Dec;19(12):530-4. doi: 10.1016/s0166-2236(96)40004-2. Trends Neurosci. 1996. PMID: 8961481 Review. - Artifactual voltage response recorded from hair cells with patch-clamp amplifiers.
Masetto S, Weng T, Valli P, Correia MJ. Masetto S, et al. Neuroreport. 1999 Jun 23;10(9):1837-41. doi: 10.1097/00001756-199906230-00007. Neuroreport. 1999. PMID: 10501517 - Computational modeling of spike generation in serotonergic neurons of the dorsal raphe nucleus.
Tuckwell HC, Penington NJ. Tuckwell HC, et al. Prog Neurobiol. 2014 Jul;118:59-101. doi: 10.1016/j.pneurobio.2014.04.001. Epub 2014 Apr 29. Prog Neurobiol. 2014. PMID: 24784445 - Influence of conductance changes on patch clamp capacitance measurements using a lock-in amplifier and limitations of the phase tracking technique.
Debus K, Hartmann J, Kilic G, Lindau M. Debus K, et al. Biophys J. 1995 Dec;69(6):2808-22. doi: 10.1016/S0006-3495(95)80154-2. Biophys J. 1995. PMID: 8599687 Free PMC article. - [Series: Utilization of Differential Equations and Methods for Solving Them in Medical Physics (2)].
Murase K. Murase K. Igaku Butsuri. 2015;35(1):49-58. Igaku Butsuri. 2015. PMID: 26753397 Review. Japanese.
Cited by
- High-throughput methods for cardiac cellular electrophysiology studies: the road to personalized medicine.
Seibertz F, Voigt N. Seibertz F, et al. Am J Physiol Heart Circ Physiol. 2024 Apr 1;326(4):H938-H949. doi: 10.1152/ajpheart.00599.2023. Epub 2024 Jan 26. Am J Physiol Heart Circ Physiol. 2024. PMID: 38276947 Review. - Amplification of input differences by dynamic heterogeneity in the spiral ganglion.
Crozier RA, Wismer ZQ, Parra-Munevar J, Plummer MR, Davis RL. Crozier RA, et al. J Neurophysiol. 2022 May 1;127(5):1317-1333. doi: 10.1152/jn.00544.2021. Epub 2022 Apr 7. J Neurophysiol. 2022. PMID: 35389760 Free PMC article. - Dynamic Heterogeneity Shapes Patterns of Spiral Ganglion Activity.
Parra-Munevar J, Morse CE, Plummer MR, Davis RL. Parra-Munevar J, et al. J Neurosci. 2021 Oct 27;41(43):8859-8875. doi: 10.1523/JNEUROSCI.0924-20.2021. Epub 2021 Sep 22. J Neurosci. 2021. PMID: 34551939 Free PMC article. - Analog transmission of action potential fine structure in spiral ganglion axons.
Liu W, Liu Q, Crozier RA, Davis RL. Liu W, et al. J Neurophysiol. 2021 Sep 1;126(3):888-905. doi: 10.1152/jn.00237.2021. Epub 2021 Aug 4. J Neurophysiol. 2021. PMID: 34346782 Free PMC article. - A hybrid stochastic/deterministic model of single photon response and light adaptation in mouse rods.
Beelen CJ, Asteriti S, Cangiano L, Koch KW, Dell'Orco D. Beelen CJ, et al. Comput Struct Biotechnol J. 2021 Jun 23;19:3720-3734. doi: 10.1016/j.csbj.2021.06.033. eCollection 2021. Comput Struct Biotechnol J. 2021. PMID: 34285774 Free PMC article.
References
- J Neurophysiol. 1992 Nov;68(5):1834-41 - PubMed
- Neuroscience. 1982 May;7(5):1233-42 - PubMed
- Neuroscience. 1993 Mar;53(1):121-30 - PubMed
- J Neurophysiol. 1993 Jul;70(1):284-98 - PubMed
- Eur J Neurosci. 1994 Mar 1;6(3):381-91 - PubMed
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources