Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing - PubMed (original) (raw)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing
R Tacke et al. Cell. 1998.
Free article
Abstract
The RNA-binding protein Tra2 is an important regulator of sex determination in Drosophila. Recently, two mammalian Tra2 homologs of unknown function have been described. Here, we show that human Tra2 proteins are present in HeLa cell nuclear extracts and that they bind efficiently and specifically to a previously characterized pre-mRNA splicing enhancer element. Indeed, both purified proteins bound preferentially to RNA sequences containing GAA repeats, characteristic of many enhancer elements. Neither Tra2 protein functioned in constitutive splicing in vitro, but both activated enhancer-dependent splicing in a sequence-specific manner and restored it after inhibition with competitor RNA. Our findings indicate that mammalian Tra2 proteins are sequence-specific splicing activators that likely participate in the control of cell-specific splicing patterns.
Similar articles
- A complex of nuclear proteins mediates SR protein binding to a purine-rich splicing enhancer.
Yeakley JM, Morfin JP, Rosenfeld MG, Fu XD. Yeakley JM, et al. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7582-7. doi: 10.1073/pnas.93.15.7582. Proc Natl Acad Sci U S A. 1996. PMID: 8755518 Free PMC article. - Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer.
Lynch KW, Maniatis T. Lynch KW, et al. Genes Dev. 1996 Aug 15;10(16):2089-101. doi: 10.1101/gad.10.16.2089. Genes Dev. 1996. PMID: 8769651 - Direct repression of splicing by transformer-2.
Chandler DS, Qi J, Mattox W. Chandler DS, et al. Mol Cell Biol. 2003 Aug;23(15):5174-85. doi: 10.1128/MCB.23.15.5174-5185.2003. Mol Cell Biol. 2003. PMID: 12861004 Free PMC article. - Functions of SR and Tra2 proteins in pre-mRNA splicing regulation.
Tacke R, Manley JL. Tacke R, et al. Proc Soc Exp Biol Med. 1999 Feb;220(2):59-63. doi: 10.1046/j.1525-1373.1999.d01-10.x. Proc Soc Exp Biol Med. 1999. PMID: 9933499 Review. No abstract available. - Tra2 protein biology and mechanisms of splicing control.
Best A, Dalgliesh C, Kheirollahi-Kouhestani M, Danilenko M, Ehrmann I, Tyson-Capper A, Elliott DJ. Best A, et al. Biochem Soc Trans. 2014 Aug;42(4):1152-8. doi: 10.1042/BST20140075. Biochem Soc Trans. 2014. PMID: 25110018 Review.
Cited by
- The Role of Splicing Factors in Adipogenesis and Thermogenesis.
Naing YT, Sun L. Naing YT, et al. Mol Cells. 2023 May 31;46(5):268-277. doi: 10.14348/molcells.2023.2195. Epub 2023 Apr 2. Mol Cells. 2023. PMID: 37170770 Free PMC article. Review. - TRA2: The dominant power of alternative splicing in tumors.
Xue J, Ma T, Zhang X. Xue J, et al. Heliyon. 2023 Apr 15;9(4):e15516. doi: 10.1016/j.heliyon.2023.e15516. eCollection 2023 Apr. Heliyon. 2023. PMID: 37151663 Free PMC article. Review. - Discovery of lipid-mediated protein-protein interactions in living cells using metabolic labeling with photoactivatable clickable probes.
Fedoryshchak RO, Gorelik A, Shen M, Shchepinova MM, Pérez-Dorado I, Tate EW. Fedoryshchak RO, et al. Chem Sci. 2023 Jan 30;14(9):2419-2430. doi: 10.1039/d2sc06116c. eCollection 2023 Mar 1. Chem Sci. 2023. PMID: 36873846 Free PMC article. - Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair.
Sigismondo G, Arseni L, Palacio-Escat N, Hofmann TG, Seiffert M, Krijgsveld J. Sigismondo G, et al. Nucleic Acids Res. 2023 Jan 25;51(2):687-711. doi: 10.1093/nar/gkac1264. Nucleic Acids Res. 2023. PMID: 36629267 Free PMC article. - Exonic splicing code and protein binding sites for calcium.
Pengelly RJ, Bakhtiar D, Borovská I, Královičová J, Vořechovský I. Pengelly RJ, et al. Nucleic Acids Res. 2022 Jun 10;50(10):5493-5512. doi: 10.1093/nar/gkac270. Nucleic Acids Res. 2022. PMID: 35474482 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous