Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation - PubMed (original) (raw)

. 1997 Dec 15;159(12):6018-29.

Affiliations

PMID: 9550400

Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation

Y Teraki et al. J Immunol. 1997.

Abstract

Although considerable attention has been paid to the development of cytokine synthesis heterogeneity during memory T cell differentiation, little information is available on how this function is coregulated with homing receptor expression. The development of skin-homing, CD4+ memory T cells in the human provides an excellent model for such investigation, since 1) the skin supports both Th1- and Th2-predominant responses in different settings, and 2) the skin-homing capability of human memory T cells correlates with and appears to depend on expression of the skin-selective homing receptor cutaneous lymphocyte-associated Ag (CLA). In this study, we used multiparameter FACS analysis to examine expression of CLA vs IFN-gamma, IL-4, and IL-2 synthesis capabilities among fresh peripheral blood CD4+ memory T cells, and Th1 vs Th2 memory T cells generated in vitro from purified CD4+ naive precursors by cyclic activation in polarizing culture conditions. Among normal peripheral blood T cells, CLA expression was essentially identical among the IFN-gamma- vs IL-4-producing CD4+ memory subsets, clearly indicating the existence of in vivo mechanisms capable of producing both Th1 vs Th2 skin-homing T cells. In vitro differentiation of naive CD4+ T cells confirmed the independent regulation of CLA and all three cytokines examined, regulation that allowed differential production of IFN-gamma-, IL-4-, and IL-2-producing, CLA+ memory subsets. These studies also 1) demonstrated differences in regulatory factor activity depending on the differentiation status of the responding cell, and 2) revealed CLA expression to be much more rapidly reversible on established memory cells than cytokine synthesis capabilities.

PubMed Disclaimer

Cited by

Lymphocyte and CD62E expression in lichen planus and lichenoid reaction.
Werneck JT, Souza Gonçalves L, Marques LC, Junior AS. Werneck JT, et al. BMC Oral Health. 2022 Nov 17;22(1):507. doi: 10.1186/s12903-022-02496-5. BMC Oral Health. 2022. PMID: 36397025 Free PMC article.
HECA-452 is a non-function blocking antibody for isolated sialyl Lewis x adhesion to endothelial expressed E-selectin under flow conditions.
Kummitha CM, Shirure VS, Delgadillo LF, Deosarkar SP, Tees DF, Burdick MM, Goetz DJ. Kummitha CM, et al. J Immunol Methods. 2012 Oct 31;384(1-2):43-50. doi: 10.1016/j.jim.2012.07.003. Epub 2012 Jul 20. J Immunol Methods. 2012. PMID: 22820001 Free PMC article.
Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
Ni Z, Walcheck B. Ni Z, et al. Clin Immunol. 2009 Nov;133(2):257-64. doi: 10.1016/j.clim.2009.07.010. Epub 2009 Aug 8. Clin Immunol. 2009. PMID: 19665434 Free PMC article.
Impaired CD4 and CD8 T cell phenotype and reduced chemokine secretion in recent-onset type 1 diabetic children.
Hedman M, Faresjö M, Axelsson S, Ludvigsson J, Casas R. Hedman M, et al. Clin Exp Immunol. 2008 Sep;153(3):360-8. doi: 10.1111/j.1365-2249.2008.03720.x. Clin Exp Immunol. 2008. PMID: 18803760 Free PMC article.
Cellular players and role of selectin ligands in leukocyte recruitment in a T-cell-initiated delayed-type hypersensitivity reaction.
Doebis C, Siegmund K, Loddenkemper C, Lowe JB, Issekutz AC, Hamann A, Huehn J, Syrbe U. Doebis C, et al. Am J Pathol. 2008 Oct;173(4):1067-76. doi: 10.2353/ajpath.2008.080052. Epub 2008 Aug 28. Am J Pathol. 2008. PMID: 18755847 Free PMC article.

Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation - PubMed (original) (raw)