Generation of Escherichia coli O9a serotype, a subtype of E. coli O9, by transfer of the wb* gene cluster of Klebsiella O3 into E. coli via recombination - PubMed (original) (raw)

Generation of Escherichia coli O9a serotype, a subtype of E. coli O9, by transfer of the wb* gene cluster of Klebsiella O3 into E. coli via recombination

T Sugiyama et al. J Bacteriol. 1998 May.

Abstract

Genetic characterization of the wb* gene in a series of Escherichia coli and Klebsiella strains possessing the mannose homopolymer as the O-specific polysaccharide was carried out. The partial nucleotide sequences and PCR-restriction fragment length polymorphism analysis suggested that E. coli serotype O9a, a subtype of E. coli O9, might have been generated by the insertion of the Klebsiella O3 wb* gene into a certain E. coli strain.

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Figures

FIG. 1

FIG. 1

Genetic map and alignment sequences around the putative recombination sites. Fragments A, B, and C, produced by primers LrfbM-1 and LwzmO9a-1, TLmtfC-1 and LmtfC-2, and LrfbKR-1 and LmtfC-2, respectively, are shown above the genetic map of the E. coli F719 serotype O9a wb* gene cluster and 3′ end of the his operon. Restriction enzyme maps of the region from manB to wbdC in Klebsiella O3 and E. coli O9a and O9 are shown below the genetic map. Nucleotides at polymorphic sites around the putative recombination sites in the connective region of the wzm and wzt genes and in the middle of the wbdC gene are displayed at the bottom. Invariant sites are not shown. Restriction sites are based on the result of the PCR-RFLP. All nucleotide sequences except those of wzm and wzt in E. coli O9a are consensus sequences of all strains analyzed in each serotype indicated. The nucleotide numbers, relative to the 5′ end of each gene, are given above the sequences. Dots indicate bases identical to those in Klebsiella O3. The nucleotides in the regions similar to those in Klebsiella O3 are boxed. Abbreviations: N, _Nhe_I; Sm, _Sma_I; St, _Stu_I; X, _Xba_I; K, G or T; M, A or C; R, A or G; S, C or G; Y, C or T.

FIG. 2

FIG. 2

Phylogenetic trees constructed with the nucleotide sequences of the 5′ (bases 1 to 445) and 3′ (bases 477 to 822) halves of wbdC (a and b, respectively), wzm (c), and wzt (d). Branch lengths are proportional to evolutionary distances. Bootstrap values are given at the nodes. O serotypes are given in parentheses. Abbreviations: EO8, E. coli O8; EO9, E. coli O9; EO9a, E. coli O9a; KO3, Klebsiella O3; KO5, Klebsiella O5.

References

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