Inductive role of fibroblastic cell lines in development of the mouse thymus anlage in organ culture - PubMed (original) (raw)
. 1998 Jan 10;183(1):32-41.
doi: 10.1006/cimm.1998.1239.
Affiliations
- PMID: 9578717
- DOI: 10.1006/cimm.1998.1239
Inductive role of fibroblastic cell lines in development of the mouse thymus anlage in organ culture
M Itoi et al. Cell Immunol. 1998.
Abstract
Previously, we have shown that embryonic day 12 thymus anlage cultured alone cannot develop into the mature organ but degenerates. In the present study, we investigated the cause of this insufficient organogenesis of embryonic day 12 thymus anlage in organ culture. We cocultured embryonic day 12 thymus anlages with various cell lines as pellets formed by centrifugation. In coculture with fibroblastic cell lines, but not with thymic epithelial cell lines, embryonic day 12 thymus anlages developed to support full T cell differentiation, and expressed mature stromal cell markers, Ia and Kb. By pellet culture of thymus anlages and fibroblastic cell lines transfected with a beta-galactosidase expression vector, we analyzed the distribution of added fibroblastic cells in pellets. The added fibroblastic cells constituted neither thymic capsule nor septa but disappeared after about 2 weeks in culture. Moreover, immunohistochemical studies indicated that added fibroblastic cells were adjacent to mesenchymal cells of thymus anlage. Our results strongly suggest that added fibroblastic cells support the development of the thymus anlage through interaction with its mesenchymal cells.
Similar articles
- Mesenchymal cells are required for functional development of thymic epithelial cells.
Itoi M, Tsukamoto N, Yoshida H, Amagai T. Itoi M, et al. Int Immunol. 2007 Aug;19(8):953-64. doi: 10.1093/intimm/dxm060. Epub 2007 Jul 11. Int Immunol. 2007. PMID: 17625108 - Limited development capacity of the earliest embryonic murine thymus.
Amagai T, Itoi M, Kondo Y. Amagai T, et al. Eur J Immunol. 1995 Mar;25(3):757-62. doi: 10.1002/eji.1830250320. Eur J Immunol. 1995. PMID: 7705405 - A novel culture system for induction of T cell development: modification of fetal thymus organ culture.
Dou YM, Watanabe Y, Aiba Y, Wada K, Katsura Y. Dou YM, et al. Thymus. 1994-1995;23(3-4):195-207. Thymus. 1994. PMID: 8525505 - Thymus organogenesis and molecular mechanisms of thymic epithelial cell differentiation.
Manley NR. Manley NR. Semin Immunol. 2000 Oct;12(5):421-8. doi: 10.1006/smim.2000.0263. Semin Immunol. 2000. PMID: 11085174 Review. - Cellular and molecular events during early thymus development.
Holländer G, Gill J, Zuklys S, Iwanami N, Liu C, Takahama Y. Holländer G, et al. Immunol Rev. 2006 Feb;209:28-46. doi: 10.1111/j.0105-2896.2006.00357.x. Immunol Rev. 2006. PMID: 16448532 Review.
Cited by
- FSP1(+) fibroblast subpopulation is essential for the maintenance and regeneration of medullary thymic epithelial cells.
Sun L, Sun C, Liang Z, Li H, Chen L, Luo H, Zhang H, Ding P, Sun X, Qin Z, Zhao Y. Sun L, et al. Sci Rep. 2015 Oct 8;5:14871. doi: 10.1038/srep14871. Sci Rep. 2015. PMID: 26445893 Free PMC article. - Differential requirement for mesenchyme in the proliferation and maturation of thymic epithelial progenitors.
Jenkinson WE, Jenkinson EJ, Anderson G. Jenkinson WE, et al. J Exp Med. 2003 Jul 21;198(2):325-32. doi: 10.1084/jem.20022135. Epub 2003 Jul 14. J Exp Med. 2003. PMID: 12860931 Free PMC article. - An essential role for thymic mesenchyme in early T cell development.
Suniara RK, Jenkinson EJ, Owen JJ. Suniara RK, et al. J Exp Med. 2000 Mar 20;191(6):1051-6. doi: 10.1084/jem.191.6.1051. J Exp Med. 2000. PMID: 10727466 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources