Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein - PubMed (original) (raw)
. 1998 May 22;273(21):13104-9.
doi: 10.1074/jbc.273.21.13104.
Affiliations
- PMID: 9582349
- DOI: 10.1074/jbc.273.21.13104
Free article
Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein
E Rom et al. J Biol Chem. 1998.
Free article
Abstract
All eukaryotic mRNAs (except organellar) are capped at their 5' end. The cap structure (m7GpppN, where N is any nucleotide) is extremely important for the processing and translation of mRNA. Several cap-binding proteins that facilitate these processes have been characterized. Here we describe a novel human cytoplasmic protein that is 30% identical and 60% similar to the human translation initiation factor 4E (eIF4E). We demonstrate that this protein, named 4E Homologous Protein (4EHP), binds specifically to capped RNA in an ATP- and divalent ion-independent manner. The three-dimensional structure of 4EHP, as predicted by homology modeling, closely resembles that of eIF4E and site-directed mutagenesis analysis of 4EHP strongly suggests that it shares with eIF4E a common mechanism for cap binding. A putative function for 4EHP is discussed.
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