Construction of YAC-based mammalian artificial chromosomes - PubMed (original) (raw)
Construction of YAC-based mammalian artificial chromosomes
M Ikeno et al. Nat Biotechnol. 1998 May.
Abstract
To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.
Comment in
- Human artificial chromosomes coming into focus.
Willard HF. Willard HF. Nat Biotechnol. 1998 May;16(5):415-6. doi: 10.1038/nbt0598-415. Nat Biotechnol. 1998. PMID: 9592384 No abstract available.
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