Activation of caspases triggered by cytochrome c in vitro - PubMed (original) (raw)
Activation of caspases triggered by cytochrome c in vitro
G Pan et al. FEBS Lett. 1998.
Free article
Erratum in
- FEBS Lett 1998 May 29;428(3):309
Abstract
Previous studies have shown that Apaf-1 and caspase-9 in the presence of cytochrome c and dATP can form an initiating complex for an apoptotic protease cascade. We have developed a cytochrome c-dependent in vitro system in which caspases downstream of this initiation complex are activated. The activation of caspase-9 from zymogen form to active dimeric protease requires intrinsic enzymatic activity. In contrast, caspase-3 and caspase-7 zymogens are proteolytically processed by active caspase-9. Activation of the above caspases is blocked by a dominant negative form of caspase-9. The in vitro system displays surprising specificity in that other caspases, including 1, 2, 4, 8, 10, and 13, are not activated.
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