Clinical evaluation of a rapid immunochromatographic test for the diagnosis of dengue virus infection - PubMed (original) (raw)

Comparative Study

Clinical evaluation of a rapid immunochromatographic test for the diagnosis of dengue virus infection

C T Sang et al. Clin Diagn Lab Immunol. 1998 May.

Abstract

A rapid immunochromatographic test was compared to the hemagglutination inhibition assay for separate determinations of dengue virus-specific immunoglobulin M (IgM) and IgG levels in paired serum specimens from 92 patients (34 with primary dengue virus infection, 35 with secondary dengue virus infection, and 23 without dengue virus infection). The rapid test showed 99% sensitivity in the diagnosis of dengue virus infection. The majority (30 of 34 [88%]) of patients with primary infection showed positive IgM but negative IgG, while 34 of 35 (97%) patients with secondary infection showed positive IgG with or without IgM. Specificity in nonflavivirus infections was 96% (1 of 23 positive). The rapid test should be a useful aid in rapid diagnosis of dengue virus infection.

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Figures

FIG. 1

FIG. 1

Inside view of the PanBio Dengue Rapid Test device, showing general instructions for use. The locations of the antigen pad, gold-labelled MAb conjugate pad, and absorbent pad are shown, as well as the anti-human (αh) IgG, anti-human IgM, control, and limit lines.

References

    1. Cardosa M J, Baharudin F, Hamid S, Hooi T P, Nimmanitya S. A nitrocellulose membrane based IgM capture enzyme immunoassay for etiological diagnosis of dengue virus infections. Clin Diagn Virol. 1995;3:343–350. - PubMed
    1. Clarke D H, Casals J. Techniques for hemagglutination and hemagglutination inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958;7:561–573. - PubMed
    1. Gubler D J. Dengue haemorrhagic fever: a global update. Virus Inf Exchange Newsl. 1991;8:2–3.
    1. Gubler D J. World distribution of dengue. Dengue Bull. 1996;20:1–4.
    1. Gubler D J. Serological diagnosis of dengue/dengue haemorrhagic fever. Dengue Bull. 1996;20:20–23.

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