Inducible gene targeting in mice using the Cre/lox system - PubMed (original) (raw)
Review
Inducible gene targeting in mice using the Cre/lox system
B Sauer. Methods. 1998 Apr.
Abstract
Molecular techniques now allow the design of precise genetic modifications in the mouse. Not only can defined nucleotide changes be engineered into the genome of the mouse, but genetic switches can be designed to target expression or ablation of any gene (for which basic molecular information is available) to any tissue at any defined time. These strategies promise to contribute substantially to an increased understanding of individual gene function in development and pathogenesis. A powerful tool, both for the design of such genetic switches and for speeding the creation of gene-modified animals, is the Cre site-specific DNA recombinase of bacteriophage P1. Precise DNA rearrangements and genetic switches can be efficiently generated in a straightforward manner using Cre recombinase. In conjunction with inducible systems for controlling Cre expression and function, these recombination-based strategies are likely to have a profound impact on developmental biology and the generation of useful animal models of human disease.
Similar articles
- Temporal control of Cre recombinase-mediated in vitro DNA recombination by Tet-on gene expression system.
Guo ZM, Xu K, Yue Y, Huang B, Deng XY, Zhong NQ, Hong X, Chen XG, Xiao D. Guo ZM, et al. Acta Biochim Biophys Sin (Shanghai). 2005 Feb;37(2):133-8. Acta Biochim Biophys Sin (Shanghai). 2005. PMID: 15685371 - Site- and time-specific gene targeting in the mouse.
Metzger D, Chambon P. Metzger D, et al. Methods. 2001 May;24(1):71-80. doi: 10.1006/meth.2001.1159. Methods. 2001. PMID: 11327805 - Conditional gene targeting in the mouse nervous system: Insights into brain function and diseases.
Gavériaux-Ruff C, Kieffer BL. Gavériaux-Ruff C, et al. Pharmacol Ther. 2007 Mar;113(3):619-34. doi: 10.1016/j.pharmthera.2006.12.003. Epub 2007 Jan 10. Pharmacol Ther. 2007. PMID: 17289150 Review. - Beta cell-specific ablation of target gene using Cre-loxP system in transgenic mice.
Ray MK, Fagan SP, Moldovan S, DeMayo FJ, Brunicardi FC. Ray MK, et al. J Surg Res. 1999 Jun 15;84(2):199-203. doi: 10.1006/jsre.1999.5642. J Surg Res. 1999. PMID: 10357920 - [Modification of gene targeting method for functional analysis of the target gene in vivo].
Shibata H, Kanamaru R, Noda T. Shibata H, et al. Gan To Kagaku Ryoho. 1997 Feb;24(4):460-5. Gan To Kagaku Ryoho. 1997. PMID: 9063484 Review. Japanese.
Cited by
- Suppression of Gq Function Using Intra-Pipette Delivery of shRNA during Extracellular Recording in the Ventral Tegmental Area.
Nimitvilai S, Arora DS, McElvain MA, Brodie MS. Nimitvilai S, et al. Front Cell Neurosci. 2013 Feb 12;7:7. doi: 10.3389/fncel.2013.00007. eCollection 2013. Front Cell Neurosci. 2013. PMID: 23408114 Free PMC article. - Protocadherins mediate dendritic self-avoidance in the mammalian nervous system.
Lefebvre JL, Kostadinov D, Chen WV, Maniatis T, Sanes JR. Lefebvre JL, et al. Nature. 2012 Aug 23;488(7412):517-21. doi: 10.1038/nature11305. Nature. 2012. PMID: 22842903 Free PMC article. - Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice.
Guzeloglu-Kayisli O, Lalioti MD, Aydiner F, Sasson I, Ilbay O, Sakkas D, Lowther KM, Mehlmann LM, Seli E. Guzeloglu-Kayisli O, et al. Biochem J. 2012 Aug 15;446(1):47-58. doi: 10.1042/BJ20120467. Biochem J. 2012. PMID: 22621333 Free PMC article. - Gene targeting in the mouse: advances in introduction of transgenes into the genome by homologous recombination.
Misra RP, Duncan SA. Misra RP, et al. Endocrine. 2002 Dec;19(3):229-38. doi: 10.1385/ENDO:19:3:229. Endocrine. 2002. PMID: 12624422 Review. - Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells.
Loonstra A, Vooijs M, Beverloo HB, Allak BA, van Drunen E, Kanaar R, Berns A, Jonkers J. Loonstra A, et al. Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9209-14. doi: 10.1073/pnas.161269798. Proc Natl Acad Sci U S A. 2001. PMID: 11481484 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources