Screening mosaic F1 females for mutations affecting zebrafish heart induction and patterning - PubMed (original) (raw)
Screening mosaic F1 females for mutations affecting zebrafish heart induction and patterning
J Alexander et al. Dev Genet. 1998.
Abstract
The genetic pathways underlying the induction and anterior-posterior patterning of the heart are poorly understood. The recent emergence of the zebrafish model system now allows a classical genetic approach to such challenging problems in vertebrate development. Two large-scale screens for mutations affecting zebrafish embryonic development have recently been completed; among the hundreds of mutations identified were several that affect specific aspects of cardiac morphogenesis, differentiation, and function. However, very few mutations affecting induction and/or anterior-posterior patterning of the heart were identified. We hypothesize that a directed approach utilizing molecular markers to examine these particular steps of heart development will uncover additional such mutations. To test this hypothesis, we are conducting two parallel screens for mutations that affect either the induction or the anterior-posterior patterning of the zebrafish heart. As an indicator of cardiac induction, we examine expression of nkx2.5, the earliest known marker of precardiac mesoderm; to assess anterior-posterior patterning, we distinguish ventricle from atrium with antibodies that recognize different myosin heavy chain isoforms. In order to expedite the examination of a large number of mutations, we are screening the haploid progeny of mosaic F1 females. In these ongoing screens, we have identified four mutations that affect nkx2.5 expression as well as 21 that disrupt either ventricular or atrial development and thus far have recovered several of these mutations, demonstrating the value of our approach. Future analysis of these and other cardiac mutations will provide further insight into the processes of induction and anterior-posterior patterning of the heart.
Similar articles
- Cardiac patterning and morphogenesis in zebrafish.
Yelon D. Yelon D. Dev Dyn. 2001 Dec;222(4):552-63. doi: 10.1002/dvdy.1243. Dev Dyn. 2001. PMID: 11748825 Review. - Patterning during organogenesis: genetic analysis of cardiac chamber formation.
Yelon D, Stainier DY. Yelon D, et al. Semin Cell Dev Biol. 1999 Feb;10(1):93-8. doi: 10.1006/scdb.1998.0278. Semin Cell Dev Biol. 1999. PMID: 10355033 Review. - Genetic analysis of tissue interactions required for otic placode induction in the zebrafish.
Mendonsa ES, Riley BB. Mendonsa ES, et al. Dev Biol. 1999 Feb 1;206(1):100-12. doi: 10.1006/dbio.1998.9134. Dev Biol. 1999. PMID: 9918698 - Somite development in zebrafish.
Stickney HL, Barresi MJ, Devoto SH. Stickney HL, et al. Dev Dyn. 2000 Nov;219(3):287-303. doi: 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1065>3.0.CO;2-A. Dev Dyn. 2000. PMID: 11066087 Review. - Restricted expression of cardiac myosin genes reveals regulated aspects of heart tube assembly in zebrafish.
Yelon D, Horne SA, Stainier DY. Yelon D, et al. Dev Biol. 1999 Oct 1;214(1):23-37. doi: 10.1006/dbio.1999.9406. Dev Biol. 1999. PMID: 10491254
Cited by
- The nutrient sensor CRTC and Sarcalumenin/thinman represent an alternate pathway in cardiac hypertrophy.
Dondi C, Vogler G, Gupta A, Walls SM, Kervadec A, Marchant J, Romero MR, Diop S, Goode J, Thomas JB, Colas AR, Bodmer R, Montminy M, Ocorr K. Dondi C, et al. Cell Rep. 2024 Aug 27;43(8):114549. doi: 10.1016/j.celrep.2024.114549. Epub 2024 Aug 1. Cell Rep. 2024. PMID: 39093699 Free PMC article. - Somatostatin signalling coordinates energy metabolism allocation to reproduction in zebrafish.
Chen J, Zhao W, Cao L, Martins RST, Canário AVM. Chen J, et al. BMC Biol. 2024 Jul 29;22(1):163. doi: 10.1186/s12915-024-01961-7. BMC Biol. 2024. PMID: 39075492 Free PMC article. - Spinetoram-Induced Potential Neurotoxicity through Autophagy Mediated by Mitochondrial Damage.
Chen F, Lu J, Li M, Yang J, Xu W, Jiang X, Zhang Y. Chen F, et al. Molecules. 2024 Jan 3;29(1):253. doi: 10.3390/molecules29010253. Molecules. 2024. PMID: 38202836 Free PMC article. - VEGFA mRNA-LNP promotes biliary epithelial cell-to-hepatocyte conversion in acute and chronic liver diseases and reverses steatosis and fibrosis.
Rizvi F, Lee YR, Diaz-Aragon R, Bawa PS, So J, Florentino RM, Wu S, Sarjoo A, Truong E, Smith AR, Wang F, Everton E, Ostrowska A, Jung K, Tam Y, Muramatsu H, Pardi N, Weissman D, Soto-Gutierrez A, Shin D, Gouon-Evans V. Rizvi F, et al. Cell Stem Cell. 2023 Dec 7;30(12):1640-1657.e8. doi: 10.1016/j.stem.2023.10.008. Epub 2023 Nov 28. Cell Stem Cell. 2023. PMID: 38029740 - Zebrafish cardiac repolarization does not functionally depend on the expression of the hERG1b-like transcript.
Genge CE, Muralidharan P, Kemp J, Hull CM, Yip M, Simpson K, Hunter DV, Claydon TW. Genge CE, et al. Pflugers Arch. 2024 Jan;476(1):87-99. doi: 10.1007/s00424-023-02875-z. Epub 2023 Nov 7. Pflugers Arch. 2024. PMID: 37934265
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials