Effects of aging on the dynamics of lymphocyte organ distribution in mice: use of a radioiodinated cell membrane probe - PubMed (original) (raw)
Effects of aging on the dynamics of lymphocyte organ distribution in mice: use of a radioiodinated cell membrane probe
J W Albright et al. Mech Ageing Dev. 1998.
Abstract
We have employed a derivatized aminostyrylpyridinium dye, [125I]I2P-Di-6-ASP, to provide a relatively stable tag on mixed mouse splenocytes and purified B and T cells for the purpose of tracking the distribution of those cells among the organs of normal young (4 months) and aged (> 26 months) recipient mice. Cells from both young and aged donor spleens were studied. Special emphasis was placed on localization of donor cells in the spleens of the recipients because the majority of circulating lymphocytes localize in the spleen and the spleen is the principal organ of primary immune response. There was a profound difference in the efficiency of splenic acquisition of donor cells between young and aged recipients, a difference not found in the liver, lungs, kidneys or heart. In contrast young and old donor lymphocytes lodged equally well in the spleens of recipients of the same age. It was clear that the competence of the splenic microenvironment to serve as a lodging site for circulating lymphocytes deteriorated with age. Such a change could contribute significantly to the deficient immune response of aged subjects. We suggest that aging results in significant change in the splenic extracellular matrix to serve as an adhesive substratum for lymphocytes. Our data point to a need for detailed studies on age-related changes in components of the extracellular matrix within lymphoid tissues. The novel compound which we employed for cell labeling is both radioactive and fluorescent and should be quite suitable for such studies.
Similar articles
- Trypanosoma musculi: tracking parasites and circulating lymphoid cells in host mice.
Albright JW, Mease RC, Lambert C, Albright JF. Albright JW, et al. Exp Parasitol. 1999 Feb;91(2):185-95. doi: 10.1006/expr.1998.4376. Exp Parasitol. 1999. PMID: 9990347 - [Age-related changes in splenic lymphocyte migration in mice].
Anokhin IuN, Iarilin AA, Norets TA. Anokhin IuN, et al. Ontogenez. 1980;11(5):511-7. Ontogenez. 1980. PMID: 7003463 Review. Russian. - Lymphocyte surface receptors and leukemia virus-induced immunosuppression.
Friedman H, Kateley JR. Friedman H, et al. Am J Clin Pathol. 1975 May;63(5):735-47. doi: 10.1093/ajcp/63.5.735. Am J Clin Pathol. 1975. PMID: 1093386 Review.
Cited by
- Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging.
Gawne PJ, Man F, Blower PJ, T M de Rosales R. Gawne PJ, et al. Chem Rev. 2022 Jun 8;122(11):10266-10318. doi: 10.1021/acs.chemrev.1c00767. Epub 2022 May 12. Chem Rev. 2022. PMID: 35549242 Free PMC article. Review. - Synthesis and Biological Evaluation of Substrate-Based Imaging Agents for the Prostate-Specific Membrane Antigen.
Byun Y, Pullambhatla M, Wang H, Mease RC, Pomper MG. Byun Y, et al. Macromol Res. 2013 May 1;21(5):565-573. doi: 10.1007/s13233-013-1050-5. Macromol Res. 2013. PMID: 25328507 Free PMC article. - Ageing, autoimmunity and arthritis: senescence of the B cell compartment - implications for humoral immunity.
Johnson SA, Cambier JC. Johnson SA, et al. Arthritis Res Ther. 2004;6(4):131-9. doi: 10.1186/ar1180. Epub 2004 May 10. Arthritis Res Ther. 2004. PMID: 15225355 Free PMC article. Review. - Effects of ageing on the immunoregulation of parasitic infection.
Humphreys NE, Grencis RK. Humphreys NE, et al. Infect Immun. 2002 Sep;70(9):5148-57. doi: 10.1128/IAI.70.9.5148-5157.2002. Infect Immun. 2002. PMID: 12183565 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical