Enhancement of strand invasion by oligonucleotides through manipulation of backbone charge - PubMed (original) (raw)

Comparative Study

. 1996 Dec;14(13):1700-4.

doi: 10.1038/nbt1296-1700.

Affiliations

• PMID: 9634855
• DOI: 10.1038/nbt1296-1700

Comparative Study

Enhancement of strand invasion by oligonucleotides through manipulation of backbone charge

S V Smulevitch et al. Nat Biotechnol. 1996 Dec.

Abstract

The ability of DNA oligonucleotides, neutral peptide nucleic acids (PNAS), and oligonucleotide conjugates to hybridize to inverted repeat sequences within supercoiled double-stranded DNA by Watson-Crick base-pairing is examined. PNAs and oligonucleotide conjugates initiate and maintain strand invasion under more stringent conditions than do unmodified DNA oligonucleotides. PNAs hybridize rapidly and, once bound, hold open a target site allowing oligonucleotides to base-pair to the displaced strand under conditions that would otherwise preclude hybridization. The ability to manipulate hybridization efficiency through different options for the alteration of oligomer charge should have important implications for optimizing sequence-specific recognition of DNA.

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Comment in

• Close encounters of the PNA kind.
  Lansdorp PM. Lansdorp PM. Nat Biotechnol. 1996 Dec;14(13):1653. doi: 10.1038/nbt1296-1653a. Nat Biotechnol. 1996. PMID: 9634843 No abstract available.

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