Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation - PubMed (original) (raw)
Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation
M D Welch et al. Science. 1998.
Abstract
Actin filament assembly at the cell surface of the pathogenic bacterium Listeria monocytogenes requires the bacterial ActA surface protein and the host cell Arp2/3 complex. Purified Arp2/3 complex accelerated the nucleation of actin polymerization in vitro, but pure ActA had no effect. However, when combined, the Arp2/3 complex and ActA synergistically stimulated the nucleation of actin filaments. This mechanism of activating the host Arp2/3 complex at the L. monocytogenes surface may be similar to the strategy used by cells to control Arp2/3 complex activity and hence the spatial and temporal distribution of actin polymerization.
Similar articles
- Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes.
Welch MD, Iwamatsu A, Mitchison TJ. Welch MD, et al. Nature. 1997 Jan 16;385(6613):265-9. doi: 10.1038/385265a0. Nature. 1997. PMID: 9000076 - Listeria protein ActA mimics WASp family proteins: it activates filament barbed end branching by Arp2/3 complex.
Boujemaa-Paterski R, Gouin E, Hansen G, Samarin S, Le Clainche C, Didry D, Dehoux P, Cossart P, Kocks C, Carlier MF, Pantaloni D. Boujemaa-Paterski R, et al. Biochemistry. 2001 Sep 25;40(38):11390-404. doi: 10.1021/bi010486b. Biochemistry. 2001. PMID: 11560487 - Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility.
Skoble J, Auerbuch V, Goley ED, Welch MD, Portnoy DA. Skoble J, et al. J Cell Biol. 2001 Oct 1;155(1):89-100. doi: 10.1083/jcb.200106061. J Cell Biol. 2001. PMID: 11581288 Free PMC article. - Actin cytoskeleton: the Arp2/3 complex gets to the point.
Zigmond SH. Zigmond SH. Curr Biol. 1998 Sep 10;8(18):R654-7. doi: 10.1016/s0960-9822(07)00415-0. Curr Biol. 1998. PMID: 9740796 Review. - Actin-based motility as a self-organized system: mechanism and reconstitution in vitro.
Carlier MF, Wiesner S, Le Clainche C, Pantaloni D. Carlier MF, et al. C R Biol. 2003 Feb;326(2):161-70. doi: 10.1016/s1631-0691(03)00067-2. C R Biol. 2003. PMID: 12754935 Review.
Cited by
- Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia.
Pizarro-Cerdá J, Charbit A, Enninga J, Lafont F, Cossart P. Pizarro-Cerdá J, et al. Semin Cell Dev Biol. 2016 Dec;60:155-167. doi: 10.1016/j.semcdb.2016.07.019. Epub 2016 Jul 19. Semin Cell Dev Biol. 2016. PMID: 27448494 Free PMC article. Review. - Three regions within ActA promote Arp2/3 complex-mediated actin nucleation and Listeria monocytogenes motility.
Skoble J, Portnoy DA, Welch MD. Skoble J, et al. J Cell Biol. 2000 Aug 7;150(3):527-38. doi: 10.1083/jcb.150.3.527. J Cell Biol. 2000. PMID: 10931865 Free PMC article. - Molecular mechanism of Arp2/3 complex inhibition by Arpin.
Fregoso FE, van Eeuwen T, Simanov G, Rebowski G, Boczkowska M, Zimmet A, Gautreau AM, Dominguez R. Fregoso FE, et al. Nat Commun. 2022 Feb 2;13(1):628. doi: 10.1038/s41467-022-28112-2. Nat Commun. 2022. PMID: 35110533 Free PMC article. - Principles of intracellular bacterial pathogen spread from cell to cell.
Weddle E, Agaisse H. Weddle E, et al. PLoS Pathog. 2018 Dec 13;14(12):e1007380. doi: 10.1371/journal.ppat.1007380. eCollection 2018 Dec. PLoS Pathog. 2018. PMID: 30543716 Free PMC article. Review. No abstract available. - A biomimetic motility assay provides insight into the mechanism of actin-based motility.
Wiesner S, Helfer E, Didry D, Ducouret G, Lafuma F, Carlier MF, Pantaloni D. Wiesner S, et al. J Cell Biol. 2003 Feb 3;160(3):387-98. doi: 10.1083/jcb.200207148. Epub 2003 Jan 27. J Cell Biol. 2003. PMID: 12551957 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous