Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes - PubMed (original) (raw)

Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes

F Crawford et al. Immunity. 1998 Jun.

Free article

Abstract

Multimeric soluble MHC class II molecules stably occupied with covalently attached peptides bind with appropriate specificity to T cell hybridomas and T cells from T cell receptor transgenic mice. There is a direct correlation between soluble T cell receptor affinity for monomeric MHC/peptide and level of binding of multimeric MHC/peptide to T cells. While binding of the multimeric MHC/peptide complex is proportional to T cell receptor affinity and expression level, there is little influence of T cell CD4.

PubMed Disclaimer

Similar articles

• Functional evidence for TCR-intrinsic specificity for MHCII.
  Parrish HL, Deshpande NR, Vasic J, Kuhns MS. Parrish HL, et al. Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3000-5. doi: 10.1073/pnas.1518499113. Epub 2016 Feb 1. Proc Natl Acad Sci U S A. 2016. PMID: 26831112 Free PMC article.
• MHC class II loading of high or low affinity peptides directed by Ii/peptide fusion constructs: implications for T cell activation.
  Gregers TF, Fleckenstein B, Vartdal F, Roepstorff P, Bakke O, Sandlie I. Gregers TF, et al. Int Immunol. 2003 Nov;15(11):1291-9. doi: 10.1093/intimm/dxg128. Int Immunol. 2003. PMID: 14565927
• MHC class II/T-cell receptor interactions potentiate secretion of IgG but not IgM in response to T-dependent antigens.
  Takahashi Y, Ametani A, Totsuka M, Fujine K, Tsuji NM, Kurisaki J, Kaminogawa S. Takahashi Y, et al. Immunol Lett. 1998 Apr;61(2-3):97-101. doi: 10.1016/s0165-2478(98)00002-9. Immunol Lett. 1998. PMID: 9657260
• Structural basis of T cell recognition of peptides bound to MHC molecules.
  Wang JH, Reinherz EL. Wang JH, et al. Mol Immunol. 2002 May;38(14):1039-49. doi: 10.1016/s0161-5890(02)00033-0. Mol Immunol. 2002. PMID: 11955596 Review.
• The T cell antigen receptor: the Swiss army knife of the immune system.
  Attaf M, Legut M, Cole DK, Sewell AK. Attaf M, et al. Clin Exp Immunol. 2015 Jul;181(1):1-18. doi: 10.1111/cei.12622. Epub 2015 May 14. Clin Exp Immunol. 2015. PMID: 25753381 Free PMC article. Review.

Cited by

• Aire mediates tolerance to insulin through thymic trimming of high-affinity T cell clones.
  Smith JA, Yuen BTK, Purtha W, Balolong JM, Phipps JD, Crawford F, Bluestone JA, Kappler JW, Anderson MS. Smith JA, et al. Proc Natl Acad Sci U S A. 2024 May 14;121(20):e2320268121. doi: 10.1073/pnas.2320268121. Epub 2024 May 6. Proc Natl Acad Sci U S A. 2024. PMID: 38709934 Free PMC article.
• Genetically engineered CD80-pMHC-harboring extracellular vesicles for antigen-specific CD4+ T-cell engagement.
  Ishina IA, Kurbatskaia IN, Mamedov AE, Shramova EI, Deyev SM, Nurbaeva KS, Rubtsov YP, Belogurov AA Jr, Gabibov AG, Zakharova MY. Ishina IA, et al. Front Bioeng Biotechnol. 2024 Jan 17;11:1341685. doi: 10.3389/fbioe.2023.1341685. eCollection 2023. Front Bioeng Biotechnol. 2024. PMID: 38304104 Free PMC article.
• The CD4+ T cell repertoire specific for citrullinated peptides shows evidence of immune tolerance.
  McElwee MK, Dileepan T, Mahmud SA, Jenkins MK. McElwee MK, et al. J Exp Med. 2023 Dec 4;220(12):e20230209. doi: 10.1084/jem.20230209. Epub 2023 Oct 13. J Exp Med. 2023. PMID: 37831103 Free PMC article.
• Insertion of an immunodominant T helper cell epitope within the Group A Streptococcus M protein promotes an IFN-γ-dependent shift from a non-protective to a protective immune response.
  Emami S, Rojas Converso T, Persson JJ, Johansson-Lindbom B. Emami S, et al. Front Immunol. 2023 Aug 15;14:1241485. doi: 10.3389/fimmu.2023.1241485. eCollection 2023. Front Immunol. 2023. PMID: 37654501 Free PMC article.
• CD4+ T cell memory.
  Künzli M, Masopust D. Künzli M, et al. Nat Immunol. 2023 Jun;24(6):903-914. doi: 10.1038/s41590-023-01510-4. Epub 2023 May 8. Nat Immunol. 2023. PMID: 37156885 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program