Site-specific ribonuclease activity of eukaryotic DNA topoisomerase I - PubMed (original) (raw)
Site-specific ribonuclease activity of eukaryotic DNA topoisomerase I
J Sekiguchi et al. Mol Cell. 1997 Dec.
Free article
Abstract
Type I topoisomerases alter DNA topology by cleaving and rejoining one strand of duplex DNA through a covalent protein-DNA intermediate. Here we show that vaccinia topoisomerase, a eukaryotic type IB enzyme, catalyzes site-specific endoribonucleolytic cleavage of an RNA-containing strand. The RNase reaction occurs via transesterification at the scissile ribonucleotide to form a covalent RNA-3'-phosphoryl-enzyme intermediate, which is then attacked by the vicinal 2' OH of the ribose sugar to yield a free 2', 3' cyclic phosphate product. Introduction of a single ribonucleoside at the scissile phosphate of an otherwise all-DNA substrate suffices to convert the topoisomerase into an endonuclease. Human topoisomerase I also has endoribonuclease activity. These findings suggest potential roles for topoisomerases in RNA processing.
Similar articles
- Site-specific DNA transesterification by vaccinia topoisomerase: role of specific phosphates and nucleosides.
Cheng C, Shuman S. Cheng C, et al. Biochemistry. 1999 Dec 14;38(50):16599-612. doi: 10.1021/bi992001d. Biochemistry. 1999. PMID: 10600122 - Ribonuclease activity of vaccinia DNA topoisomerase IB: kinetic and high-throughput inhibition studies using a robust continuous fluorescence assay.
Kwon K, Nagarajan R, Stivers JT. Kwon K, et al. Biochemistry. 2004 Nov 30;43(47):14994-5004. doi: 10.1021/bi048801s. Biochemistry. 2004. PMID: 15554707 - Polynucleotide ligase activity of eukaryotic topoisomerase I.
Shuman S. Shuman S. Mol Cell. 1998 Apr;1(5):741-8. doi: 10.1016/s1097-2765(00)80073-8. Mol Cell. 1998. PMID: 9660957 - Vaccinia virus DNA topoisomerase: a model eukaryotic type IB enzyme.
Shuman S. Shuman S. Biochim Biophys Acta. 1998 Oct 1;1400(1-3):321-37. doi: 10.1016/s0167-4781(98)00144-4. Biochim Biophys Acta. 1998. PMID: 9748643 Review. - Structure of DNA topoisomerases.
Berger JM. Berger JM. Biochim Biophys Acta. 1998 Oct 1;1400(1-3):3-18. doi: 10.1016/s0167-4781(98)00124-9. Biochim Biophys Acta. 1998. PMID: 9748476 Review.
Cited by
- Genome Instability Induced by Topoisomerase Misfunction.
Nitiss KC, Bandak A, Berger JM, Nitiss JL. Nitiss KC, et al. Int J Mol Sci. 2024 Sep 24;25(19):10247. doi: 10.3390/ijms251910247. Int J Mol Sci. 2024. PMID: 39408578 Free PMC article. Review. - From the TOP: Formation, recognition and resolution of topoisomerase DNA protein crosslinks.
Wojtaszek JL, Williams RS. Wojtaszek JL, et al. DNA Repair (Amst). 2024 Oct;142:103751. doi: 10.1016/j.dnarep.2024.103751. Epub 2024 Aug 16. DNA Repair (Amst). 2024. PMID: 39180935 Review. - The Last Universal Common Ancestor of Ribosome-Encoding Organisms: Portrait of LUCA.
Forterre P. Forterre P. J Mol Evol. 2024 Oct;92(5):550-583. doi: 10.1007/s00239-024-10186-9. Epub 2024 Aug 19. J Mol Evol. 2024. PMID: 39158619 Review. - Deletions initiated by the vaccinia virus TopIB protein in yeast.
Cho JE, Shaltz S, Yakovleva L, Shuman S, Jinks-Robertson S. Cho JE, et al. DNA Repair (Amst). 2024 May;137:103664. doi: 10.1016/j.dnarep.2024.103664. Epub 2024 Mar 6. DNA Repair (Amst). 2024. PMID: 38484460 - The C-Terminal Acid Phosphatase Module of the RNase HI Enzyme RnhC Controls Rifampin Sensitivity and Light-Dependent Colony Pigmentation of Mycobacterium smegmatis.
Dupuy P, Glickman MS. Dupuy P, et al. J Bacteriol. 2023 Apr 25;205(4):e0043122. doi: 10.1128/jb.00431-22. Epub 2023 Mar 14. J Bacteriol. 2023. PMID: 36916909 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials