Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin - PubMed (original) (raw)
Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin
G Mizuguchi et al. Mol Cell. 1997 Dec.
Free article
Abstract
The Drosophila nucleosome remodeling factor (NURF) is a protein complex of four subunits that assists transcription factor-mediated perturbation of nucleosomes in an ATP-dependent manner. We have investigated the role of NURF in activating transcription from a preassembled chromatin template and have found that NURF is able to facilitate transcription mediated by a GAL4 derivative carrying both a DNA binding and an activator domain. Interestingly, once nucleosome remodeling by the DNA binding factor is accomplished, a high level of NURF activity is not continuously required for recruitment of the general transcriptional machinery and transcription for at least 100 nucleotides. Our results provide direct evidence that NURF is able to assist gene activation in a chromatin context, and identify a stage of NURF dependence early in the process leading to transcriptional initiation.
Similar articles
- Biological functions of the ISWI chromatin remodeling complex NURF.
Badenhorst P, Voas M, Rebay I, Wu C. Badenhorst P, et al. Genes Dev. 2002 Dec 15;16(24):3186-98. doi: 10.1101/gad.1032202. Genes Dev. 2002. PMID: 12502740 Free PMC article. - GAL4 directs nucleosome sliding induced by NURF.
Kang JG, Hamiche A, Wu C. Kang JG, et al. EMBO J. 2002 Mar 15;21(6):1406-13. doi: 10.1093/emboj/21.6.1406. EMBO J. 2002. PMID: 11889046 Free PMC article. - Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators.
Kwon SY, Grisan V, Jang B, Herbert J, Badenhorst P. Kwon SY, et al. PLoS Genet. 2016 Apr 5;12(4):e1005969. doi: 10.1371/journal.pgen.1005969. eCollection 2016 Apr. PLoS Genet. 2016. PMID: 27046080 Free PMC article. - ATP-dependent remodeling of chromatin.
Wu C, Tsukiyama T, Gdula D, Georgel P, Martínez-Balbás M, Mizuguchi G, Ossipow V, Sandaltzopoulos R, Wang HM. Wu C, et al. Cold Spring Harb Symp Quant Biol. 1998;63:525-34. doi: 10.1101/sqb.1998.63.525. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384317 Review. No abstract available. - SWItched-on mobility.
Guschin D, Wolffe AP. Guschin D, et al. Curr Biol. 1999 Oct 7;9(19):R742-6. doi: 10.1016/s0960-9822(99)80473-4. Curr Biol. 1999. PMID: 10530996 Review.
Cited by
- Genome-Wide Identification of Long Noncoding RNA and Their Potential Interactors in ISWI Mutants.
Zhang L, Zhang S, Wang R, Sun L. Zhang L, et al. Int J Mol Sci. 2022 Jun 2;23(11):6247. doi: 10.3390/ijms23116247. Int J Mol Sci. 2022. PMID: 35682924 Free PMC article. - Dual Regulatory Role of Chromatin Remodeler ISW1 in Coordinating Cellulase and Secondary Metabolite Biosynthesis in Trichoderma reesei.
Cao Y, Yang R, Zheng F, Meng X, Zhang W, Liu W. Cao Y, et al. mBio. 2021 Feb 22;13(1):e0345621. doi: 10.1128/mbio.03456-21. Epub 2022 Feb 8. mBio. 2021. PMID: 35130719 Free PMC article. - Opportunity knocks for uncovering the new function of an understudied nucleosome remodeling complex member, the bromodomain PHD finger transcription factor, BPTF.
Zahid H, Olson NM, Pomerantz WCK. Zahid H, et al. Curr Opin Chem Biol. 2021 Aug;63:57-67. doi: 10.1016/j.cbpa.2021.02.003. Epub 2021 Mar 8. Curr Opin Chem Biol. 2021. PMID: 33706239 Free PMC article. Review. - Chromatin remodelers: We are the drivers!!
Tyagi M, Imam N, Verma K, Patel AK. Tyagi M, et al. Nucleus. 2016 Jul 3;7(4):388-404. doi: 10.1080/19491034.2016.1211217. Epub 2016 Jul 18. Nucleus. 2016. PMID: 27429206 Free PMC article. Review. - Epigenomic regulation of oncogenesis by chromatin remodeling.
Kumar R, Li DQ, Müller S, Knapp S. Kumar R, et al. Oncogene. 2016 Aug 25;35(34):4423-36. doi: 10.1038/onc.2015.513. Epub 2016 Jan 25. Oncogene. 2016. PMID: 26804164 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases