Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS - PubMed (original) (raw)

Comparative Study

. 1998 Jul 3;430(3):419-23.

doi: 10.1016/s0014-5793(98)00706-6.

Affiliations

• PMID: 9688584
• DOI: 10.1016/s0014-5793(98)00706-6

Free article

Comparative Study

Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS

N J Clarke et al. FEBS Lett. 1998.

Free article

Abstract

A quantitative method for detection of amyloid beta peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of A beta 1-40 and A beta 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for A beta 1-40 and A beta 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other A beta peptide species that may also play a role in the onset of AD in humans.

PubMed Disclaimer

Similar articles

• Analysis of in vivo-derived amyloid-beta polypeptides by on-line two-dimensional chromatography-mass spectrometry.
  Clarke NJ, Crow FW, Younkin S, Naylor S. Clarke NJ, et al. Anal Biochem. 2001 Nov 1;298(1):32-9. doi: 10.1006/abio.2001.5357. Anal Biochem. 2001. PMID: 11673892
• High-performance liquid chromatography coupled with tandem mass spectrometry for the detection of amyloid Beta peptide related with Alzheimer's disease.
  Du C, Ramaley C, McLean H, Leonard SC, Miller J. Du C, et al. J Biomol Tech. 2005 Dec;16(4):356-63. J Biomol Tech. 2005. PMID: 16522857 Free PMC article.
• Quantification of Alzheimer amyloid beta peptides ending at residues 40 and 42 by novel ELISA systems.
  Jensen M, Hartmann T, Engvall B, Wang R, Uljon SN, Sennvik K, Näslund J, Muehlhauser F, Nordstedt C, Beyreuther K, Lannfelt L. Jensen M, et al. Mol Med. 2000 Apr;6(4):291-302. Mol Med. 2000. PMID: 10949910 Free PMC article.
• Quantitative detection of amyloid-β peptides by mass spectrometry: state of the art and clinical applications.
  Bros P, Delatour V, Vialaret J, Lalere B, Barthelemy N, Gabelle A, Lehmann S, Hirtz C. Bros P, et al. Clin Chem Lab Med. 2015 Sep 1;53(10):1483-93. doi: 10.1515/cclm-2014-1048. Clin Chem Lab Med. 2015. PMID: 25719328 Review.
• The use of mass spectrometry to study amyloid-β peptides.
  Grasso G. Grasso G. Mass Spectrom Rev. 2011 May-Jun;30(3):347-65. doi: 10.1002/mas.20281. Epub 2010 Nov 22. Mass Spectrom Rev. 2011. PMID: 21500241 Review.

Cited by

• Colorimetric cellulose-based test-strip for rapid detection of amyloid β-42.
  Moreira FTC, Correia BP, Sousa MP, Sales GF. Moreira FTC, et al. Mikrochim Acta. 2021 Sep 8;188(10):334. doi: 10.1007/s00604-021-04996-7. Mikrochim Acta. 2021. PMID: 34498145
• 4-Hexylresorcinol-in duced angiogenesis potential in human endothelial cells.
  Kim MK, Kim SG, Lee SK. Kim MK, et al. Maxillofac Plast Reconstr Surg. 2020 Jun 29;42(1):23. doi: 10.1186/s40902-020-00267-2. eCollection 2020 Dec. Maxillofac Plast Reconstr Surg. 2020. PMID: 32642459 Free PMC article.
• γ-Secretase-Dependent Proteolysis of Transmembrane Domain of Amyloid Precursor Protein: Successive Tri- and Tetrapeptide Release in Amyloid β-Protein Production.
  Takami M, Funamoto S. Takami M, et al. Int J Alzheimers Dis. 2012;2012:591392. doi: 10.1155/2012/591392. Epub 2012 Dec 31. Int J Alzheimers Dis. 2012. PMID: 23346458 Free PMC article.
• Effects of 4-Hexylresorcinol on Protein Expressions in RAW 264.7 Cells as Determined by Immunoprecipitation High Performance Liquid Chromatography.
  Kim MK, Yoon CS, Kim SG, Park YW, Lee SS, Lee SK. Kim MK, et al. Sci Rep. 2019 Mar 4;9(1):3379. doi: 10.1038/s41598-019-38946-4. Sci Rep. 2019. PMID: 30833641 Free PMC article.
• Development of a magnetic immunosorbent for on-chip preconcentration of amyloid β isoforms: Representatives of Alzheimer's disease biomarkers.
  Svobodova Z, Reza Mohamadi M, Jankovicova B, Esselmann H, Verpillot R, Otto M, Taverna M, Wiltfang J, Viovy JL, Bilkova Z. Svobodova Z, et al. Biomicrofluidics. 2012 Jun;6(2):24126-2412612. doi: 10.1063/1.4722588. Epub 2012 May 24. Biomicrofluidics. 2012. PMID: 22712037 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database