Complex structure of knob DNA on maize chromosome 9. Retrotransposon invasion into heterochromatin - PubMed (original) (raw)

Complex structure of knob DNA on maize chromosome 9. Retrotransposon invasion into heterochromatin

E V Ananiev et al. Genetics. 1998 Aug.

Abstract

The recovery of maize (Zea mays L.) chromosome addition lines of oat (Avena sativa L.) from oat x maize crosses enables us to analyze the structure and composition of specific regions, such as knobs, of individual maize chromosomes. A DNA hybridization blot panel of eight individual maize chromosome addition lines revealed that 180-bp repeats found in knobs are present in each of these maize chromosomes, but the copy number varies from approximately 100 to 25, 000. Cosmid clones with knob DNA segments were isolated from a genomic library of an oat-maize chromosome 9 addition line with the help of the 180-bp knob-associated repeated DNA sequence used as a probe. Cloned knob DNA segments revealed a complex organization in which blocks of tandemly arranged 180-bp repeating units are interrupted by insertions of other repeated DNA sequences, mostly represented by individual full size copies of retrotransposable elements. There is an obvious preference for the integration of retrotransposable elements into certain sites (hot spots) of the 180-bp repeat. Sequence microheterogeneity including point mutations and duplications was found in copies of 180-bp repeats. The 180-bp repeats within an array all had the same polarity. Restriction maps constructed for 23 cloned knob DNA fragments revealed the positions of polymorphic sites and sites of integration of insertion elements. Discovery of the interspersion of retrotransposable elements among blocks of tandem repeats in maize and some other organisms suggests that this pattern may be basic to heterochromatin organization for eukaryotes.

PubMed Disclaimer

Cited by

Intraspecific violation of genetic colinearity and its implications in maize.
Fu H, Dooner HK. Fu H, et al. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9573-8. doi: 10.1073/pnas.132259199. Epub 2002 Jun 11. Proc Natl Acad Sci U S A. 2002. PMID: 12060715 Free PMC article.
Transposons and genome evolution in plants.
Fedoroff N. Fedoroff N. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7002-7. doi: 10.1073/pnas.97.13.7002. Proc Natl Acad Sci U S A. 2000. PMID: 10860963 Free PMC article. Review.
A maize homolog of mammalian CENPC is a constitutive component of the inner kinetochore.
Dawe RK, Reed LM, Yu HG, Muszynski MG, Hiatt EN. Dawe RK, et al. Plant Cell. 1999 Jul;11(7):1227-38. doi: 10.1105/tpc.11.7.1227. Plant Cell. 1999. PMID: 10402425 Free PMC article.
Unbiased K-mer Analysis Reveals Changes in Copy Number of Highly Repetitive Sequences During Maize Domestication and Improvement.
Liu S, Zheng J, Migeon P, Ren J, Hu Y, He C, Liu H, Fu J, White FF, Toomajian C, Wang G. Liu S, et al. Sci Rep. 2017 Feb 10;7:42444. doi: 10.1038/srep42444. Sci Rep. 2017. PMID: 28186206 Free PMC article.
Molecular, genetic and evolutionary analysis of a paracentric inversion in Arabidopsis thaliana.
Fransz P, Linc G, Lee CR, Aflitos SA, Lasky JR, Toomajian C, Ali H, Peters J, van Dam P, Ji X, Kuzak M, Gerats T, Schubert I, Schneeberger K, Colot V, Martienssen R, Koornneef M, Nordborg M, Juenger TE, de Jong H, Schranz ME. Fransz P, et al. Plant J. 2016 Oct;88(2):159-178. doi: 10.1111/tpj.13262. Epub 2016 Sep 13. Plant J. 2016. PMID: 27436134 Free PMC article.

References

1. Genetics. 1995 Feb;139(2):659-70 - PubMed
1. Genetics. 1929 Mar;14(2):180-222 - PubMed
1. Chromosoma. 1991 Dec;101(3):141-56 - PubMed
1. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3539-43 - PubMed
1. Anal Biochem. 1984 Feb;137(1):266-7 - PubMed

Complex structure of knob DNA on maize chromosome 9. Retrotransposon invasion into heterochromatin - PubMed (original) (raw)