11Beta-hydroxysteroid dehydrogenase, mineralocorticoid receptor, and thiazide-sensitive Na-Cl cotransporter expression by distal tubules - PubMed (original) (raw)
11Beta-hydroxysteroid dehydrogenase, mineralocorticoid receptor, and thiazide-sensitive Na-Cl cotransporter expression by distal tubules
M Bostanjoglo et al. J Am Soc Nephrol. 1998 Aug.
Erratum in
- J Am Soc Nephrol 1998 Nov;9(11):2179. Bostonjoglo M [corrected to Bostanjoglo M]
Abstract
Mineralocorticoid hormones regulate salt transport along the distal nephron by binding to intracellular receptors and activating gene transcription. Previous experiments showed that systemic aldosterone infusions stimulate thiazide-sensitive Na and Cl transport by distal convoluted tubule (DCT) cells; this effect could have been direct or secondary to systemic hormonal effects. Aldosterone target tissues express both mineralocorticoid receptors and the metabolic enzyme 11beta-hydroxysteroid dehydrogenase type 2. Mineralocorticoid receptors have been localized to the DCT in some experiments, but not in others. Expression of 11beta-hydroxysteroid dehydrogenase type 2 by DCT cells has not been investigated. The present experiments were designed to test the hypothesis that rat DCT cells are targets of aldosterone action. Patterns of mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase, thiazide-sensitive Na-Cl cotransporter, and Na/Ca exchanger expression along the distal tubule were examined. A polyclonal antibody was generated to localize the thiazide-sensitive Na-Cl cotransporter. Thiazide-sensitive Na-Cl cotransporter and 11beta-hydroxysteroid dehydrogenase expression were examined using both in situ hybridization and immunocytochemistry; Na/Ca exchanger and mineralocorticoid receptor expression were examined by immunocytochemistry. The results indicate that 11beta-hydroxysteroid dehydrogenase is expressed by DCT cells, as well as connecting tubule cells and principal cells of the collecting duct; expression levels are low near the junction with the thick ascending limb and rise near the transition to the connecting tubule. Mineralocorticoid receptors are expressed by DCT cells, as well as along the thick ascending limb, connecting tubule, and collecting duct. The results indicate that components of the mineralocorticoid receptor system are expressed by DCT cells, suggesting that these cells are targets of aldosterone action.
Similar articles
- Localization of thiazide-sensitive Na(+)-Cl(-) cotransport and associated gene products in mouse DCT.
Câmpean V, Kricke J, Ellison D, Luft FC, Bachmann S. Câmpean V, et al. Am J Physiol Renal Physiol. 2001 Dec;281(6):F1028-35. doi: 10.1152/ajprenal.0148.2001. Am J Physiol Renal Physiol. 2001. PMID: 11704553 - Rabbit distal convoluted tubule coexpresses NaCl cotransporter and 11 beta-hydroxysteroid dehydrogenase II mRNA.
Velázquez H, Náray-Fejes-Tóth A, Silva T, Andújar E, Reilly RF, Desir GV, Ellison DH. Velázquez H, et al. Kidney Int. 1998 Aug;54(2):464-72. doi: 10.1046/j.1523-1755.1998.00036.x. Kidney Int. 1998. PMID: 9690213 - Developmental expression of sodium entry pathways in rat nephron.
Schmitt R, Ellison DH, Farman N, Rossier BC, Reilly RF, Reeves WB, Oberbäumer I, Tapp R, Bachmann S. Schmitt R, et al. Am J Physiol. 1999 Mar;276(3):F367-81. doi: 10.1152/ajprenal.1999.276.3.F367. Am J Physiol. 1999. PMID: 10070160 - Regulation of distal tubule sodium transport: mechanisms and roles in homeostasis and pathophysiology.
Pearce D, Manis AD, Nesterov V, Korbmacher C. Pearce D, et al. Pflugers Arch. 2022 Aug;474(8):869-884. doi: 10.1007/s00424-022-02732-5. Epub 2022 Jul 27. Pflugers Arch. 2022. PMID: 35895103 Free PMC article. Review. - The role of 11beta-hydroxysteroid dehydrogenase in steroid hormone specificity.
Náray-Fejes-Tóth A, Colombowala IK, Fejes-Tóth G. Náray-Fejes-Tóth A, et al. J Steroid Biochem Mol Biol. 1998 Apr;65(1-6):311-6. doi: 10.1016/s0960-0760(98)00009-0. J Steroid Biochem Mol Biol. 1998. PMID: 9699885 Review.
Cited by
- Collecting duct intercalated cell function and regulation.
Roy A, Al-bataineh MM, Pastor-Soler NM. Roy A, et al. Clin J Am Soc Nephrol. 2015 Feb 6;10(2):305-24. doi: 10.2215/CJN.08880914. Epub 2015 Jan 28. Clin J Am Soc Nephrol. 2015. PMID: 25632105 Free PMC article. Review. - Single-Nucleus RNA Sequencing Reveals Loss of Distal Convoluted Tubule 1 Renal Tubules in HIV Viral Protein R Transgenic Mice.
Latt KZ, Yoshida T, Shrivastav S, Abedini A, Reece JM, Sun Z, Lee H, Okamoto K, Dagur P, Ishimoto Y, Heymann J, Zhao Y, Chung JY, Hewitt S, Jose PA, Lee K, He JC, Winkler CA, Knepper MA, Kino T, Rosenberg AZ, Susztak K, Kopp JB. Latt KZ, et al. Am J Pathol. 2024 Oct;194(10):1844-1856. doi: 10.1016/j.ajpath.2024.06.006. Epub 2024 Jul 18. Am J Pathol. 2024. PMID: 39032602 - Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.
Gonzalez-Vicente A, Saez F, Monzon CM, Asirwatham J, Garvin JL. Gonzalez-Vicente A, et al. Physiol Rev. 2019 Jan 1;99(1):235-309. doi: 10.1152/physrev.00055.2017. Physiol Rev. 2019. PMID: 30354966 Free PMC article. Review. - Distribution and regulation of expression of serum- and glucocorticoid-induced kinase-1 in the rat kidney.
Alvarez de la Rosa D, Coric T, Todorovic N, Shao D, Wang T, Canessa CM. Alvarez de la Rosa D, et al. J Physiol. 2003 Sep 1;551(Pt 2):455-66. doi: 10.1113/jphysiol.2003.042903. Epub 2003 Jun 19. J Physiol. 2003. PMID: 12816971 Free PMC article. - Mice lacking γENaC palmitoylation sites maintain benzamil-sensitive Na+ transport despite reduced channel activity.
Nickerson AJ, Mutchler SM, Sheng S, Cox NA, Ray EC, Kashlan OB, Carattino MD, Marciszyn AL, Winfrey A, Gingras S, Kirabo A, Hughey RP, Kleyman TR. Nickerson AJ, et al. JCI Insight. 2023 Nov 8;8(21):e172051. doi: 10.1172/jci.insight.172051. JCI Insight. 2023. PMID: 37707951 Free PMC article.