Circularization of mRNA by eukaryotic translation initiation factors - PubMed (original) (raw)
Circularization of mRNA by eukaryotic translation initiation factors
S E Wells et al. Mol Cell. 1998 Jul.
Free article
Abstract
Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor eIF4G. Here, we demonstrate the reconstitution of an eIF4E/eIF4G/Pab1p complex with recombinant proteins, and show by atomic force microscopy that the complex can circularize capped, polyadenylated RNA. Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell.
Similar articles
- RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G.
Kessler SH, Sachs AB. Kessler SH, et al. Mol Cell Biol. 1998 Jan;18(1):51-7. doi: 10.1128/MCB.18.1.51. Mol Cell Biol. 1998. PMID: 9418852 Free PMC article. - Binding of eukaryotic translation initiation factor 4E (eIF4E) to eIF4G represses translation of uncapped mRNA.
Tarun SZ Jr, Sachs AB. Tarun SZ Jr, et al. Mol Cell Biol. 1997 Dec;17(12):6876-86. doi: 10.1128/MCB.17.12.6876. Mol Cell Biol. 1997. PMID: 9372919 Free PMC article. - The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation.
Fortes P, Inada T, Preiss T, Hentze MW, Mattaj IW, Sachs AB. Fortes P, et al. Mol Cell. 2000 Jul;6(1):191-6. Mol Cell. 2000. PMID: 10949040 - Eukaryotic translation initiation: there are (at least) two sides to every story.
Sachs AB, Varani G. Sachs AB, et al. Nat Struct Biol. 2000 May;7(5):356-61. doi: 10.1038/75120. Nat Struct Biol. 2000. PMID: 10802729 Review.
Cited by
- Pleiotropic effects of PAB1 deletion: Extensive changes in the yeast proteome, transcriptome, and translatome.
Mangkalaphiban K, Ganesan R, Jacobson A. Mangkalaphiban K, et al. PLoS Genet. 2024 Sep 5;20(9):e1011392. doi: 10.1371/journal.pgen.1011392. eCollection 2024 Sep. PLoS Genet. 2024. PMID: 39236083 Free PMC article. - hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis.
Zare A, Salehi S, Bader J, Schneider C, Fischer U, Veh A, Arampatzi P, Mann M, Briese M, Sendtner M. Zare A, et al. Nat Commun. 2024 Aug 28;15(1):7430. doi: 10.1038/s41467-024-51678-y. Nat Commun. 2024. PMID: 39198412 Free PMC article. - Direct observation of translational activation by a ribonucleoprotein granule.
Chen R, Stainier W, Dufourt J, Lagha M, Lehmann R. Chen R, et al. Nat Cell Biol. 2024 Aug;26(8):1322-1335. doi: 10.1038/s41556-024-01452-5. Epub 2024 Jul 4. Nat Cell Biol. 2024. PMID: 38965420 Free PMC article. - Base preference for inosine 3'-riboendonuclease activity of human endonuclease V: implications for cleavage of poly-A tails containing inosine.
Mitsuoka K, Kim JI, Yoshida A, Matsumoto A, Aoki-Shioi N, Iwai S, Kuraoka I. Mitsuoka K, et al. Sci Rep. 2024 Jun 28;14(1):14973. doi: 10.1038/s41598-024-65814-7. Sci Rep. 2024. PMID: 38951658 Free PMC article. - Ribonuclease inhibitor and angiogenin system regulates cell type-specific global translation.
Stillinovic M, Sarangdhar MA, Andina N, Tardivel A, Greub F, Bombaci G, Ansermet C, Zatti M, Saha D, Xiong J, Sagae T, Yokogawa M, Osawa M, Heller M, Keogh A, Keller I, Angelillo-Scherrer A, Allam R. Stillinovic M, et al. Sci Adv. 2024 May 31;10(22):eadl0320. doi: 10.1126/sciadv.adl0320. Epub 2024 May 31. Sci Adv. 2024. PMID: 38820160 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous