Genotypic characterization of clarithromycin-resistant and -susceptible Helicobacter pylori strains from the same patient demonstrates existence of two unrelated isolates - PubMed (original) (raw)

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Genotypic characterization of clarithromycin-resistant and -susceptible Helicobacter pylori strains from the same patient demonstrates existence of two unrelated isolates

G Wang et al. J Clin Microbiol. 1998 Sep.

Abstract

Clarithromycin-susceptible and clarithromycin-resistant Helicobacter pylori isolates from the same patient were investigated for the mode of development and mechanism of clarithromycin resistance. The clarithromycin-resistant strain UA1182 harbors homozygous A-to-G mutations at position 2143 in both copies of the 23S rRNA gene and has a phenotype of resistance to clarithromycin and clindamycin but no significant resistance to streptogramin B. Pulsed-field gel electrophoresis patterns of NruI- and NotI-digested genomic DNA from the Clas and Clar isolates demonstrated that they are genetically distinct, suggesting that the development of clarithromycin resistance is not from the mutation of the existing Clas strain but from a completely new strain.

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Figures

FIG. 1

PFGE patterns of _Nru_I (lanes 1 and 3)- and _Not_I (lanes 2 and 4)-digested genomic DNA from H. pylori UA799 (lanes 1 and 2) and UA1182 (lanes 3 and 4). The sizes of lambda DNA ladder standards (lanes M) are indicated on the right.

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Genotypic characterization of clarithromycin-resistant and -susceptible Helicobacter pylori strains from the same patient demonstrates existence of two unrelated isolates - PubMed (original) (raw)