Ionic mechanism of electroresponsiveness in cerebellar granule cells implicates the action of a persistent sodium current - PubMed (original) (raw)
. 1998 Aug;80(2):493-503.
doi: 10.1152/jn.1998.80.2.493.
Affiliations
- PMID: 9705445
- DOI: 10.1152/jn.1998.80.2.493
Free article
Ionic mechanism of electroresponsiveness in cerebellar granule cells implicates the action of a persistent sodium current
E D'Angelo et al. J Neurophysiol. 1998 Aug.
Free article
Abstract
Although substantial knowledge has been accumulated on cerebellar granule cell voltage-dependent currents, their role in regulating electroresponsiveness has remained speculative. In this paper, we have used patch-clamp recording techniques in acute slice preparations to investigate the ionic basis of electroresponsiveness of rat cerebellar granule cells at a mature developmental stage. The granule cell generated a Na+-dependent spike discharge resistant to voltage and time inactivation, showing a linear frequency increase with injected currents. Action potentials arose when subthreshold depolarizing potentials, which were driven by a persistent Na+ current, reached a critical threshold. The stability and linearity of the repetitive discharge was based on a complex mechanism involving a N-type Ca2+ current blocked by omega-CTx GVIA, and a Ca2+-dependent K+ current blocked by charibdotoxin and low tetraethylammonium (TEA; <1 mM); a voltage-dependent Ca2+-independent K+ current blocked by high TEA (>1 mM); and an A current blocked by 2 mM 4-aminopyridine. Weakening TEA-sensitive K+ currents switched the granule cell into a bursting mode sustained by the persistent Na+ current. A dynamic model is proposed in which the Na+ current-dependent action potential causes secondary Ca2+ current activation and feedback voltage- and Ca2+-dependent afterhyperpolarization. The afterhyperpolarization reprimes the channels inactivated in the spike, preventing adaptation and bursting and controlling the duration of the interspike interval and firing frequency. This result reveals complex dynamics behind repetitive spike discharge and suggests that a persistent Na+ current plays an important role in action potential initiation and in the regulation of mossy fiber-granule cells transmission.
Similar articles
- Synaptic activation of Ca2+ action potentials in immature rat cerebellar granule cells in situ.
D'Angelo E, De Filippi G, Rossi P, Taglietti V. D'Angelo E, et al. J Neurophysiol. 1997 Sep;78(3):1631-42. doi: 10.1152/jn.1997.78.3.1631. J Neurophysiol. 1997. PMID: 9310448 - Endogenous pacemaker activity of rat tumour somatotrophs.
Kwiecien R, Robert C, Cannon R, Vigues S, Arnoux A, Kordon C, Hammond C. Kwiecien R, et al. J Physiol. 1998 May 1;508 ( Pt 3)(Pt 3):883-905. doi: 10.1111/j.1469-7793.1998.883bp.x. J Physiol. 1998. PMID: 9518740 Free PMC article. - Calcium spikes and calcium currents in neurons from the medial preoptic nucleus of rat.
Sundgren-Andersson AK, Johansson S. Sundgren-Andersson AK, et al. Brain Res. 1998 Feb 9;783(2):194-209. doi: 10.1016/s0006-8993(97)01342-5. Brain Res. 1998. PMID: 9507126 - Calcium current activated by potassium ions in voltage-clamped rat hippocampal pyramidal neurones.
Deák F, Nagy G, Várnai P, Madarász E, Spät A. Deák F, et al. J Physiol. 1998 May 1;508 ( Pt 3)(Pt 3):735-45. doi: 10.1111/j.1469-7793.1998.735bp.x. J Physiol. 1998. PMID: 9518729 Free PMC article. - Characteristics of action potentials and their underlying outward currents in rat taste receptor cells.
Chen Y, Sun XD, Herness S. Chen Y, et al. J Neurophysiol. 1996 Feb;75(2):820-31. doi: 10.1152/jn.1996.75.2.820. J Neurophysiol. 1996. PMID: 8714655
Cited by
- Simulations predict differing phase responses to excitation vs. inhibition in theta-resonant pyramidal neurons.
Kelley C, Antic SD, Carnevale NT, Kubie JL, Lytton WW. Kelley C, et al. J Neurophysiol. 2023 Oct 1;130(4):910-924. doi: 10.1152/jn.00160.2023. Epub 2023 Aug 23. J Neurophysiol. 2023. PMID: 37609720 Free PMC article. - Developmental timing-dependent organization of synaptic connections between mossy fibers and granule cells in the cerebellum.
Kim T, Park H, Tanaka-Yamamoto K, Yamamoto Y. Kim T, et al. Commun Biol. 2023 Apr 24;6(1):446. doi: 10.1038/s42003-023-04825-y. Commun Biol. 2023. PMID: 37095324 Free PMC article. - Computational models of neurotransmission at cerebellar synapses unveil the impact on network computation.
Masoli S, Rizza MF, Tognolina M, Prestori F, D'Angelo E. Masoli S, et al. Front Comput Neurosci. 2022 Oct 28;16:1006989. doi: 10.3389/fncom.2022.1006989. eCollection 2022. Front Comput Neurosci. 2022. PMID: 36387305 Free PMC article. Review. - Functional Neuroanatomy of the Rat Nucleus Incertus-Medial Septum Tract: Implications for the Cell-Specific Control of the Septohippocampal Pathway.
Szlaga A, Sambak P, Trenk A, Gugula A, Singleton CE, Drwiega G, Blasiak T, Ma S, Gundlach AL, Blasiak A. Szlaga A, et al. Front Cell Neurosci. 2022 Feb 25;16:836116. doi: 10.3389/fncel.2022.836116. eCollection 2022. Front Cell Neurosci. 2022. PMID: 35281300 Free PMC article. - Chronic Ethanol Exposure Enhances Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission via GluN2A Receptors in the Mouse Cerebellar Cortex.
Li BX, Dong GH, Li HL, Zhang JS, Bing YH, Chu CP, Cui SB, Qiu DL. Li BX, et al. Front Syst Neurosci. 2021 Aug 2;15:657884. doi: 10.3389/fnsys.2021.657884. eCollection 2021. Front Syst Neurosci. 2021. PMID: 34408633 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous