Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis - PubMed (original) (raw)
Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis
A F Dernburg et al. Cell. 1998.
Free article
Abstract
Chromosome segregation at meiosis I depends on pairing and crossing-over between homologs. In most eukaryotes, pairing culminates with formation of the proteinaceous synaptonemal complex (SC). In budding yeast, recombination initiates through double-strand DNA breaks (DSBs) and is thought to be essential for SC formation. Here, we examine whether this mechanism for initiating meiotic recombination is conserved, and we test the dependence of homologous chromosome synapsis on recombination in C. elegans. We find that a homolog of the yeast DSB-generating enzyme, Spo11p, is required for meiotic exchange in this metazoan, and that radiation-induced breaks partially alleviate this dependence. Thus, initiation of recombination by DSBs is apparently conserved. However, homologous synapsis is independent of recombination in the nematode, since it occurs normally in a C. elegans spo-11 null mutant.
Similar articles
- Synaptonemal Complex Central Region Proteins Promote Localization of Pro-crossover Factors to Recombination Events During Caenorhabditis elegans Meiosis.
Cahoon CK, Helm JM, Libuda DE. Cahoon CK, et al. Genetics. 2019 Oct;213(2):395-409. doi: 10.1534/genetics.119.302625. Epub 2019 Aug 20. Genetics. 2019. PMID: 31431470 Free PMC article. - Chromosome sites play dual roles to establish homologous synapsis during meiosis in C. elegans.
MacQueen AJ, Phillips CM, Bhalla N, Weiser P, Villeneuve AM, Dernburg AF. MacQueen AJ, et al. Cell. 2005 Dec 16;123(6):1037-50. doi: 10.1016/j.cell.2005.09.034. Cell. 2005. PMID: 16360034 Free PMC article. - Synapsis-defective mutants reveal a correlation between chromosome conformation and the mode of double-strand break repair during Caenorhabditis elegans meiosis.
Smolikov S, Eizinger A, Hurlburt A, Rogers E, Villeneuve AM, Colaiácovo MP. Smolikov S, et al. Genetics. 2007 Aug;176(4):2027-33. doi: 10.1534/genetics.107.076968. Epub 2007 Jun 11. Genetics. 2007. PMID: 17565963 Free PMC article. - Meiotic recombination in Caenorhabditis elegans.
Garcia-Muse T, Boulton SJ. Garcia-Muse T, et al. Chromosome Res. 2007;15(5):607-21. doi: 10.1007/s10577-007-1146-x. Chromosome Res. 2007. PMID: 17674149 Review. - Homologue pairing, recombination and segregation in Caenorhabditis elegans.
Zetka M. Zetka M. Genome Dyn. 2009;5:43-55. doi: 10.1159/000166618. Genome Dyn. 2009. PMID: 18948706 Review.
Cited by
- The conserved ATPase PCH-2 controls the number and distribution of crossovers by antagonizing crossover formation in C. elegans.
Patel B, Grobler M, Herrera A, Logari E, Ortiz V, Bhalla N. Patel B, et al. bioRxiv [Preprint]. 2024 Aug 13:2024.08.13.607819. doi: 10.1101/2024.08.13.607819. bioRxiv. 2024. PMID: 39185160 Free PMC article. Preprint. - Formation and resolution of meiotic chromosome entanglements and interlocks.
Olaya I, Burgess SM, Rog O. Olaya I, et al. J Cell Sci. 2024 Jul 1;137(13):jcs262004. doi: 10.1242/jcs.262004. Epub 2024 Jul 10. J Cell Sci. 2024. PMID: 38985540 Review. - Torenia sp. Extracts Contain Multiple Potent Antitumor Compounds with Nematocidal Activity, Triggering an Activated DNA Damage Checkpoint and Defective Meiotic Progression.
Meng Q, Borris RP, Kim HM. Meng Q, et al. Pharmaceuticals (Basel). 2024 May 10;17(5):611. doi: 10.3390/ph17050611. Pharmaceuticals (Basel). 2024. PMID: 38794181 Free PMC article. - Analysis of meiotic recombination in Drosophila simulans shows no evidence of an interchromosomal effect.
Man B, Kim E, Vadlakonda A, Stern DL, Crown KN. Man B, et al. Genetics. 2024 Aug 7;227(4):iyae084. doi: 10.1093/genetics/iyae084. Genetics. 2024. PMID: 38762892 Free PMC article. - Modeling homologous chromosome recognition via nonspecific interactions.
Marshall WF, Fung JC. Marshall WF, et al. Proc Natl Acad Sci U S A. 2024 May 14;121(20):e2317373121. doi: 10.1073/pnas.2317373121. Epub 2024 May 9. Proc Natl Acad Sci U S A. 2024. PMID: 38722810 Free PMC article.