Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans - PubMed (original) (raw)
Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans
Y Tabuse et al. Development. 1998 Sep.
Abstract
Asymmetric cell divisions, critically important to specify cell types in the development of multicellular organisms, require polarized distribution of cytoplasmic components and the proper alignment of the mitotic apparatus. In Caenorhabditis elegans, the maternally expressed protein, PAR-3, is localized to one pole of asymmetrically dividing blastomeres and is required for these asymmetric divisions. In this paper, we report that an atypical protein kinase C (PKC-3) is essential for proper asymmetric cell divisions and co-localizes with PAR-3. Embryos depleted of PKC-3 by RNA interference die showing Par-like phenotypes including defects in early asymmetric divisions and mislocalized germline-specific granules (P granules). The defective phenotypes of PKC-3-depleted embryos are similar to those exhibited by mutants for par-3 and another par gene, par-6. Direct interaction of PKC-3 with PAR-3 is shown by in vitro binding analysis. This result is reinforced by the observation that PKC-3 and PAR-3 co-localize in vivo. Furthermore, PKC-3 and PAR-3 show mutual dependence on each other and on three of the other par genes for their localization. We conclude that PKC-3 plays an indispensable role in establishing embryonic polarity through interaction with PAR-3.
Similar articles
- CDC-42 controls early cell polarity and spindle orientation in C. elegans.
Gotta M, Abraham MC, Ahringer J. Gotta M, et al. Curr Biol. 2001 Apr 3;11(7):482-8. doi: 10.1016/s0960-9822(01)00142-7. Curr Biol. 2001. PMID: 11412997 - Asymmetrically distributed PAR-3 protein contributes to cell polarity and spindle alignment in early C. elegans embryos.
Etemad-Moghadam B, Guo S, Kemphues KJ. Etemad-Moghadam B, et al. Cell. 1995 Dec 1;83(5):743-52. doi: 10.1016/0092-8674(95)90187-6. Cell. 1995. PMID: 8521491 - PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos.
Hung TJ, Kemphues KJ. Hung TJ, et al. Development. 1999 Jan;126(1):127-35. doi: 10.1242/dev.126.1.127. Development. 1999. PMID: 9834192 - Protein kinase C isotypes in C. elegans.
Tabuse Y. Tabuse Y. J Biochem. 2002 Oct;132(4):519-22. doi: 10.1093/oxfordjournals.jbchem.a003251. J Biochem. 2002. PMID: 12359064 Review. - Establishment of initial asymmetry in early Caenorhabditis elegans embryos.
Priess JR. Priess JR. Curr Opin Genet Dev. 1994 Aug;4(4):563-8. doi: 10.1016/0959-437x(94)90073-c. Curr Opin Genet Dev. 1994. PMID: 7950325 Review.
Cited by
- Optimized PAR-2 RING dimerization mediates cooperative and selective membrane binding for robust cell polarity.
Bland T, Hirani N, Briggs DC, Rossetto R, Ng K, Taylor IA, McDonald NQ, Zwicker D, Goehring NW. Bland T, et al. EMBO J. 2024 Aug;43(15):3214-3239. doi: 10.1038/s44318-024-00123-3. Epub 2024 Jun 21. EMBO J. 2024. PMID: 38907033 Free PMC article. - Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in the C. elegans zygote.
Packer J, Gubieda AG, Brooks A, Deutz LN, Squires I, Ellison S, Schneider C, Naganathan SR, Wollman AJM, Dickinson DJ, Rodriguez J. Packer J, et al. bioRxiv [Preprint]. 2024 Jun 14:2023.10.27.563985. doi: 10.1101/2023.10.27.563985. bioRxiv. 2024. PMID: 38009101 Free PMC article. Preprint. - Temporally distinct roles of Aurora A in polarization of the C. elegans zygote.
Manzi NI, de Jesus BN, Shi Y, Dickinson DJ. Manzi NI, et al. bioRxiv [Preprint]. 2023 Oct 26:2023.10.25.563816. doi: 10.1101/2023.10.25.563816. bioRxiv. 2023. PMID: 37961467 Free PMC article. Updated. Preprint. - Apical PAR protein caps orient the mitotic spindle in C. elegans early embryos.
Stolpner NJ, Manzi NI, Su T, Dickinson DJ. Stolpner NJ, et al. Curr Biol. 2023 Oct 23;33(20):4312-4329.e6. doi: 10.1016/j.cub.2023.08.069. Epub 2023 Sep 19. Curr Biol. 2023. PMID: 37729910 - How molecular techniques are developed from natural systems.
Ronai I. Ronai I. Genetics. 2023 Jul 6;224(3):iyad067. doi: 10.1093/genetics/iyad067. Genetics. 2023. PMID: 37184565 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases