Only the N-terminal domain of FtsK functions in cell division - PubMed (original) (raw)

Only the N-terminal domain of FtsK functions in cell division

G C Draper et al. J Bacteriol. 1998 Sep.

Abstract

Deletion of ftsK results in the inhibition of cell division, but this inhibition can be reversed by a plasmid carrying only the first approximately 17% of ftsK. The division block can be suppressed in most mutants by deletion of dacA, which codes for the D-alanine:D-alanine carboxypeptidase PBP5, or in all mutants by overexpression of ftsN. Overexpression of ftsK inhibits cell division and the formation of FtsZ rings. This division block is not due to the induction of either the SOS or the heat shock regulons.

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Figures

FIG. 1

FIG. 1

Cloned DNA from the trxB-lrp-ftsK-lolA region. The gene arrangement and selected restriction sites are shown at the top, with (below) the approximate locations of known promoters (filled triangles) and a putative promoter (unfilled triangle). Cloned DNA fragments are shown, together with the relative positions and orientations of controllable promoters (P_lac_ and P_BAD_) (arrowheads). Plasmid designations are shown to the right of each cloned fragment, together with the ability of each plasmid to complement the ftsK44 temperature-sensitive phenotype.

FIG. 2

FIG. 2

Inhibition of cell division by induction of ftsK. (A) MG1655/pBADK cells at 37°C in LB broth plus glucose. (B) MG1655/pBADK cells after 4 h at 37°C in LB broth plus arabinose.

FIG. 3

FIG. 3

Disruptions and deletions of the chromosomal ftsK gene (shaded) in strains CDK1, CDK2, and CDK5. Broken lines connect corresponding parts of the wild-type (WT) ftsK gene in different constructions. The approximate locations of promoters are shown as triangles (the unfilled triangles represent the repressed promoter P_dinH_).

FIG. 4

FIG. 4

Effect of disruption or partial deletion of ftsK on cell division. (A) CDK1 (ftsK::cat-1) cells in LB broth. (B and C) CDK2 (ftsK::cat-Δ2) Δ_pcnB_/pBADK cells after 2 h in LB broth plus arabinose (B) or LB broth plus glucose (C).

FIG. 5

FIG. 5

(A to C) Restoration of cell division in CDK5 (ftsK::cat-Δ5) Δ_pcnB_ cells in LB broth plus arabinose with plasmid pBADK (A), pBADK′ (B), or pBADK′3 (C). (D) CDK5 Δ_pcnB_/pBADK cells in LB broth plus glucose.

FIG. 6

FIG. 6

Overproduction of FtsN restores division in cells lacking ftsK. CDK5 (ftsK::cat-Δ5)/pKD140 (ftsN+) cells were grown in LB broth.

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