The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer's patches - PubMed (original) (raw)

. 1998 Sep 1;161(5):2449-56.

Affiliations

PMID: 9725243

The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer's patches

E J Kunkel et al. J Immunol. 1998.

Abstract

Lymphocyte trafficking into Peyer's patches requires beta 7 integrins and L-selectin. Here, we use intravital microscopy to examine leukocyte rolling and adhesion in Peyer's patch high endothelial venules (HEV) of wild-type, L-selectin-deficient (L-/-), beta 7 integrin-deficient (beta 7-/-), and beta 7/L(-/-) mice. Although the leukocyte rolling flux fraction was reduced by 70%, Peyer's patches in L-/- mice were of normal size and cellularity. In beta 7-/- mice, the rolling flux fraction was normal, but the number of adherent leukocytes in HEV was greatly reduced. The median leukocyte rolling velocity was reduced in L-/- mice and increased in beta 7-/- mice, suggesting that beta 7 integrins and L-selectin mediate rolling in Peyer's patch HEV at different velocities. beta 7/L(-/-) exhibited both a low rolling flux fraction and low adhesion and had severely reduced Peyer's patch size and cellularity. The residual rolling in these mice was completely blocked by a P-selectin mAb. A significant P-selectin component was also detected in the other genotypes. Twenty-six percent of B and T lymphocytes isolated from Peyer's patches of wild-type mice expressed functional ligands for P-selectin, and this fraction was increased to 57% in beta 7/L(-/-) mice. Peyer's patch HEV were found to express P-selectin under the conditions of intravital microscopy, but not in situ. Our data suggest a novel P-selectin dependent mechanism of lymphocyte homing to Peyer's patches. In situ, beta 7 integrins and L-selectin account for all lymphocyte homing to Peyer's patches, but P-selectin-dependent rolling, as induced by minimal trauma, may support trafficking of effector T lymphocytes to Peyer's patches.

PubMed Disclaimer

Cited by

Immune Responses to IAV Infection and the Roles of L-Selectin and ADAM17 in Lymphocyte Homing.
Reed SG, Ager A. Reed SG, et al. Pathogens. 2022 Jan 25;11(2):150. doi: 10.3390/pathogens11020150. Pathogens. 2022. PMID: 35215094 Free PMC article. Review.
Glycocalyx Degradation Induces a Proinflammatory Phenotype and Increased Leukocyte Adhesion in Cultured Endothelial Cells under Flow.
McDonald KK, Cooper S, Danielzak L, Leask RL. McDonald KK, et al. PLoS One. 2016 Dec 1;11(12):e0167576. doi: 10.1371/journal.pone.0167576. eCollection 2016. PLoS One. 2016. PMID: 27907146 Free PMC article.
Mechanism of action and efficacy of RX-111, a thieno[2,3-c]pyridine derivative and small molecule inhibitor of protein interaction with glycosaminoglycans (SMIGs), in delayed-type hypersensitivity, TNBS-induced colitis and experimental autoimmune encephalomyelitis.
Harris N, Koppel J, Zsila F, Juhas S, Il'kova G, Kogan FY, Lahmy O, Wildbaum G, Karin N, Zhuk R, Gregor P. Harris N, et al. Inflamm Res. 2016 Apr;65(4):285-94. doi: 10.1007/s00011-016-0915-4. Epub 2016 Jan 21. Inflamm Res. 2016. PMID: 26794621
Role of the endothelial surface layer in neutrophil recruitment.
Marki A, Esko JD, Pries AR, Ley K. Marki A, et al. J Leukoc Biol. 2015 Oct;98(4):503-15. doi: 10.1189/jlb.3MR0115-011R. Epub 2015 May 15. J Leukoc Biol. 2015. PMID: 25979432 Free PMC article. Review.
Smad4 promotes differentiation of effector and circulating memory CD8 T cells but is dispensable for tissue-resident memory CD8 T cells.
Hu Y, Lee YT, Kaech SM, Garvy B, Cauley LS. Hu Y, et al. J Immunol. 2015 Mar 1;194(5):2407-14. doi: 10.4049/jimmunol.1402369. Epub 2015 Jan 30. J Immunol. 2015. PMID: 25637015 Free PMC article.

The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer's patches - PubMed (original) (raw)