Comparative mapping of cloned human and murine antithyroglobulin antibodies: recognition by human antibodies of an immunodominant region - PubMed (original) (raw)

Comparative Study

Comparative mapping of cloned human and murine antithyroglobulin antibodies: recognition by human antibodies of an immunodominant region

V Estienne et al. Thyroid. 1998 Aug.

Abstract

Antibodies (Ab) to thyroglobulin (Tg) are common in patients with autoimmune thyroid diseases, but it is currently unclear how Tg Ab are involved in the pathology of autoimmune thyroid disease. We have previously reported the isolation of immunoglobulin G (IgG)kappa and IgGlambda Fab from phage display combinatorial libraries from the cervical lymph node of a single Hashimoto's thyroiditis patient with a high anti-Tg titer. Sequence analysis of these Fab indicated a restricted heavy chain usage with a nonrestricted light chain usage, with Fab inhibiting the binding of patient Tg Ab by between 39% and 79%. Comparative mapping of nine each of these IgGkappa and IgGlambda Fab, and the patient serum from whom the Fab were derived, is described here, using a panel of 10 murine monoclonal antibodies (Mab) to human thyroglobulin (hTg). The Fab interacted principally with mAb defining the overlapping antigenic domains I and IV, previously characterized as the region recognized by the majority of patient serum Tg Ab. Tg Ab from serum of the patient from whom the Fab were derived were also directed at this region, suggesting that the Fab are representative of the Tg Ab present in this patient.

PubMed Disclaimer

Cited by

Epitope recognition patterns of thyroglobulin antibody in sera from patients with Hashimoto's thyroiditis on different thyroid functional status.
Liu M, Zhao L, Gao Y, Huang Y, Lu G, Guo X. Liu M, et al. Clin Exp Immunol. 2012 Dec;170(3):283-90. doi: 10.1111/j.1365-2249.2012.04666.x. Clin Exp Immunol. 2012. PMID: 23121669 Free PMC article.
Thyroglobulin as an autoantigen: what can we learn about immunopathogenicity from the correlation of antigenic properties with protein structure?
Gentile F, Conte M, Formisano S. Gentile F, et al. Immunology. 2004 May;112(1):13-25. doi: 10.1111/j.1365-2567.2004.01861.x. Immunology. 2004. PMID: 15096179 Free PMC article. Review.
Thyroid Autoantibodies Display both "Original Antigenic Sin" and Epitope Spreading.
McLachlan SM, Rapoport B. McLachlan SM, et al. Front Immunol. 2017 Dec 20;8:1845. doi: 10.3389/fimmu.2017.01845. eCollection 2017. Front Immunol. 2017. PMID: 29326719 Free PMC article. Review.
Thyroid autoimmune disease: demonstration of thyroid antigen-specific B cells and recombination-activating gene expression in chemokine-containing active intrathyroidal germinal centers.
Armengol MP, Juan M, Lucas-Martín A, Fernández-Figueras MT, Jaraquemada D, Gallart T, Pujol-Borrell R. Armengol MP, et al. Am J Pathol. 2001 Sep;159(3):861-73. doi: 10.1016/S0002-9440(10)61762-2. Am J Pathol. 2001. PMID: 11549579 Free PMC article.
Thyroglobulin autoantibodies switch to immunoglobulin (Ig)G1 and IgG3 subclasses and preserve their restricted epitope pattern after 131I treatment for Graves' hyperthyroidism: the activity of autoimmune disease influences subclass distribution but not epitope pattern of autoantibodies.
Latrofa F, Ricci D, Montanelli L, Piaggi P, Mazzi B, Bianchi F, Brozzi F, Santini P, Fiore E, Marinò M, Tonacchera M, Vitti P. Latrofa F, et al. Clin Exp Immunol. 2014 Dec;178(3):438-46. doi: 10.1111/cei.12438. Clin Exp Immunol. 2014. PMID: 25134846 Free PMC article.

Comparative mapping of cloned human and murine antithyroglobulin antibodies: recognition by human antibodies of an immunodominant region - PubMed (original) (raw)