Vitamin D3 and its synthetic analogue secocholestra-trien-1,2, 24-triol influence the metabolism and the isomerization of retinoic acid in human keratinocytes - PubMed (original) (raw)
. 1998 May-Jun;11(3):161-5.
doi: 10.1159/000029822.
Affiliations
- PMID: 9745143
- DOI: 10.1159/000029822
Vitamin D3 and its synthetic analogue secocholestra-trien-1,2, 24-triol influence the metabolism and the isomerization of retinoic acid in human keratinocytes
F K Jugert et al. Skin Pharmacol Appl Skin Physiol. 1998 May-Jun.
Abstract
Vitamin D and vitamin A acid share metabolic pathways thereby influencing their benefit as a given drug. Little is known concerning their metabolic interactions in epidermal cells. We compared the influence of 1,25-dihydroxycholecalciferol (vitamin D3 - VD3) and its synthetic analogue secocholestra-trien-1,3,24-triol (tacalcitol - TAC) in combination with different calcium concentrations (Ca) on the metabolism and the isomerization of retinoic acid (RA) in cultured primary human keratinocytes. After preincubation with 0.09, 0.6 and 1.2 mM Ca for 24 h, followed by the addition of 10(-6), 10(-8) or 10(-10) M VD3 or TAC, we added 10(-5) M 13-cis-RA (isotretinoin). 24 h later, concentrations of RA isomers and oxidated RA metabolites were measured by RP-HPLC. VD3 (10(-6) M) inhibited the isomerization of 13-cis-RA to all-trans-RA (tretinoin) and 9-cis-RA independently from the Ca concentration in the culture medium. 10(-6)-10(-10) M TAC equally inhibit the 4-hydroxylation of all-trans-RA significantly (12.8 vs. 6.7% of total RA), thereby reducing the amount of irreversible inactivated 4-oxo-all-trans-RA, leading to a higher persistence of all-trans-RA, the active hormone. Both VD3 and its analogue TAC influence the metabolism of RA, a well-known regulator of epithelial cell proliferation and differentiation processes, in two distinct ways. Further studies are necessary to test the hypothesis that the hormone activity of RA can be increased by concomitant treatment with VD3 which prolongs the persistence of 13-cis-RA, and TAC elevating the intracellular concentration of all-trans-RA.
Similar articles
- 13-cis retinoic acid exerts its specific activity on human sebocytes through selective intracellular isomerization to all-trans retinoic acid and binding to retinoid acid receptors.
Tsukada M, Schröder M, Roos TC, Chandraratna RA, Reichert U, Merk HF, Orfanos CE, Zouboulis CC. Tsukada M, et al. J Invest Dermatol. 2000 Aug;115(2):321-7. doi: 10.1046/j.1523-1747.2000.00066.x. J Invest Dermatol. 2000. PMID: 10951254 - Vitamin D analogs affect the uptake and metabolism of retinol by human epidermal keratinocytes in culture.
Törmä H, Rollman O, Binderup L, Michaelsson G. Törmä H, et al. J Investig Dermatol Symp Proc. 1996 Apr;1(1):49-53. J Investig Dermatol Symp Proc. 1996. PMID: 9627692 - Chemically induced isomerization and differential uptake modulate retinoic acid disposition in HL-60 cells.
Lanvers C, Hempel G, Blaschke G, Boos J. Lanvers C, et al. FASEB J. 1998 Dec;12(15):1627-33. doi: 10.1096/fasebj.12.15.1627. FASEB J. 1998. PMID: 9837852 - Teratogenicity of isotretinoin revisited: species variation and the role of all-trans-retinoic acid.
Nau H. Nau H. J Am Acad Dermatol. 2001 Nov;45(5):S183-7. doi: 10.1067/mjd.2001.113720. J Am Acad Dermatol. 2001. PMID: 11606951 Review. - Vitamin A and D in allergy: from experimental animal models and cellular studies to human disease.
Hufnagl K, Jensen-Jarolim E. Hufnagl K, et al. Allergo J Int. 2018;27(3):72-78. doi: 10.1007/s40629-018-0054-2. Epub 2018 Feb 20. Allergo J Int. 2018. PMID: 29707474 Free PMC article. Review.
Cited by
- Increased integrity of cell-cell junctions accompanied by increased expression of claudin 4 in keratinocytes stimulated with vitamin D3.
Minowa E, Kurashige Y, Islam ST, Yoshida K, Sakakibara S, Okada Y, Fujita Y, Bolortsetseg D, Murai Y, Abiko Y, Saitoh M. Minowa E, et al. Med Mol Morphol. 2021 Dec;54(4):346-355. doi: 10.1007/s00795-021-00299-1. Epub 2021 Jul 29. Med Mol Morphol. 2021. PMID: 34324049 - Endogenous retinoids in the hair follicle and sebaceous gland.
Everts HB. Everts HB. Biochim Biophys Acta. 2012 Jan;1821(1):222-9. doi: 10.1016/j.bbalip.2011.08.017. Epub 2011 Sep 3. Biochim Biophys Acta. 2012. PMID: 21914489 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Medical