Resistance to Fas-mediated apoptosis: activation of caspase 3 is regulated by cell cycle regulator p21WAF1 and IAP gene family ILP - PubMed (original) (raw)
. 1998 Aug 27;17(8):931-9.
doi: 10.1038/sj.onc.1202021.
Affiliations
- PMID: 9747872
- DOI: 10.1038/sj.onc.1202021
Resistance to Fas-mediated apoptosis: activation of caspase 3 is regulated by cell cycle regulator p21WAF1 and IAP gene family ILP
A Suzuki et al. Oncogene. 1998.
Abstract
The death receptor Fas transduces apoptotic death signaling mediated by caspases. In the present study, human hepatoma HepG2 cells showed the Fas-mediated apoptosis mediated by caspase, especially caspase 3, only in the presence of actinomycin D. Interestingly, cytosolic proteins extracted from intact HepG2 cells induced caspase 3 inactivation. Our results reveal that this inactivation was triggered by the direct inhibition of activated caspase 3 by IAP gene family ILP. In addition, a 53 kDa protein was co-immunoprecipitated with anti-human caspase 3 antibody from intact HepG2 cells. This protein was a complex-protein of procaspase 3 and the cell cycle regulator p21WAF1 (p21). P21 bound to only procaspase 3, but not to activated caspase 3. We also demonstrate that p21 protein-loaded HepG2 cells resist to Fas-mediated apoptosis even in the presence of actinomycin D. Here we report that caspase 3 inactivation for the resistance to Fas-mediated apoptosis is induced by a procaspase 3/p21 complex formation and direct inhibition of activated caspase 3 by ILP.
Similar articles
- Caspase 3 inactivation to suppress Fas-mediated apoptosis: identification of binding domain with p21 and ILP and inactivation machinery by p21.
Suzuki A, Tsutomi Y, Miura M, Akahane K. Suzuki A, et al. Oncogene. 1999 Feb 4;18(5):1239-44. doi: 10.1038/sj.onc.1202409. Oncogene. 1999. PMID: 10022130 - Procaspase 3/p21 complex formation to resist fas-mediated cell death is initiated as a result of the phosphorylation of p21 by protein kinase A.
Suzuki A, Kawano H, Hayashida M, Hayasaki Y, Tsutomi Y, Akahane K. Suzuki A, et al. Cell Death Differ. 2000 Aug;7(8):721-8. doi: 10.1038/sj.cdd.4400706. Cell Death Differ. 2000. PMID: 10918446 - Survivin initiates procaspase 3/p21 complex formation as a result of interaction with Cdk4 to resist Fas-mediated cell death.
Suzuki A, Ito T, Kawano H, Hayashida M, Hayasaki Y, Tsutomi Y, Akahane K, Nakano T, Miura M, Shiraki K. Suzuki A, et al. Oncogene. 2000 Mar 2;19(10):1346-53. doi: 10.1038/sj.onc.1203429. Oncogene. 2000. PMID: 10713676 - Proteases in Fas-mediated apoptosis.
Zhivotovsky B, Burgess DH, Schlegel J, Pörn MI, Vanags D, Orrenius S. Zhivotovsky B, et al. J Cell Biochem. 1997 Jan;64(1):43-9. J Cell Biochem. 1997. PMID: 9015753 Review. - Do CTL kill target cells by inducing apoptosis?
Henkart PA, Williams MS, Zacharchuk CM, Sarin A. Henkart PA, et al. Semin Immunol. 1997 Apr;9(2):135-44. doi: 10.1006/smim.1997.0063. Semin Immunol. 1997. PMID: 9194224 Review.
Cited by
- Stress- and Rho-activated ZO-1-associated nucleic acid binding protein binding to p21 mRNA mediates stabilization, translation, and cell survival.
Nie M, Balda MS, Matter K. Nie M, et al. Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10897-902. doi: 10.1073/pnas.1118822109. Epub 2012 Jun 18. Proc Natl Acad Sci U S A. 2012. PMID: 22711822 Free PMC article. - An Elk transcription factor is required for Runx-dependent survival signaling in the sea urchin embryo.
Rizzo F, Coffman JA, Arnone MI. Rizzo F, et al. Dev Biol. 2016 Aug 1;416(1):173-186. doi: 10.1016/j.ydbio.2016.05.026. Epub 2016 May 24. Dev Biol. 2016. PMID: 27235147 Free PMC article. - BRCA1 may modulate neuronal cell cycle re-entry in Alzheimer disease.
Evans TA, Raina AK, Delacourte A, Aprelikova O, Lee HG, Zhu X, Perry G, Smith MA. Evans TA, et al. Int J Med Sci. 2007 May 12;4(3):140-5. doi: 10.7150/ijms.4.140. Int J Med Sci. 2007. PMID: 17505559 Free PMC article. - Host biomarkers and biological pathways that are associated with the expression of experimental cerebral malaria in mice.
Oakley MS, McCutchan TF, Anantharaman V, Ward JM, Faucette L, Erexson C, Mahajan B, Zheng H, Majam V, Aravind L, Kumar S. Oakley MS, et al. Infect Immun. 2008 Oct;76(10):4518-29. doi: 10.1128/IAI.00525-08. Epub 2008 Jul 21. Infect Immun. 2008. PMID: 18644885 Free PMC article. - A Functional Mutation in KIAA1462 Promoter Decreases Glucocorticoid Receptor Affinity and Affects Egg-Laying Performance in Yangzhou Geese.
Xia M, Wei W, Jiang Z, He D, Li Z, Yu S, Wang Q, Liu H, Chen J. Xia M, et al. Int J Mol Sci. 2018 May 21;19(5):1531. doi: 10.3390/ijms19051531. Int J Mol Sci. 2018. PMID: 29883426 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous