Identification and characterization of a novel member of the fibroblast growth factor family - PubMed (original) (raw)

. 1998 May;10(5):1911-25.

doi: 10.1046/j.1460-9568.1998.00211.x.

Y L Li, P A Yourey, J Gruber, K C Carter, B K Shell, P A Dillon, C Florence, D R Duan, A Blunt, D M Ornitz, S M Ruben, R F Alderson

Affiliations

• PMID: 9751161
• DOI: 10.1046/j.1460-9568.1998.00211.x

Identification and characterization of a novel member of the fibroblast growth factor family

J M Greene et al. Eur J Neurosci. 1998 May.

Abstract

A new member of the fibroblast growth factor (FGF) family, FGF-13, has been molecularly cloned as a result of high throughput sequencing of a human ovarian cancer cell library. The open reading frame of the novel human gene (1419 bp) encodes for a protein of 216 a.a. with a molecular weight of 22 kDa. The FGF-13 sequence contains an amino-terminal hydrophobic region of 23 a.a. characteristic of a signal secretion sequence. FGF-13 is most homologous, 70% similarity at the amino acid level, to FGF-8. Northern hybridization analysis demonstrated prominent expression of FGF-13 in human foetal and adult brain, particularly in the cerebellum and cortex. In proliferation studies with BaF3 cells, FGF-13 preferentially activates cell clones expressing either FGF receptor variant, 3-IIIc or 4. The signal transduction pathways of FGF-13 and FGF-2 were compared in rat hippocampal astrocytes. The two FGFs induce an equivalent level of tyrosine phosphorylation of mitogen-activated protein kinase (MAPK) and c-raf activation. However, FGF-13 is more effective than FGF-2 in inducing the phosphorylation of phospholipase C-gamma (PLC-gamma). Treatment of neuronal cultures from rat embryonic cortex with FGF-13 increases the number of glutamic acid decarboxylase immunopositive neurons, the level of high-affinity gamma-aminobutyric acid (GABA) uptake, and choline acetyltransferase enzyme activity. The GABAergic neuronal response to FGF-13 treatment is rapid with a significant increase occurring within 72 h. We have identified a novel member of the FGF family that is expressed in the central nervous system (CNS) and increases the number as well as the level of phenotypic differentiation of cortical neurons in vitro.

PubMed Disclaimer

Similar articles

• Fibroblast growth factor (FGF) receptor 1-IIIb is a naturally occurring functional receptor for FGFs that is preferentially expressed in the skin and the brain.
  Beer HD, Vindevoghel L, Gait MJ, Revest JM, Duan DR, Mason I, Dickson C, Werner S. Beer HD, et al. J Biol Chem. 2000 May 26;275(21):16091-7. doi: 10.1074/jbc.275.21.16091. J Biol Chem. 2000. PMID: 10821861
• Structure and expression of human fibroblast growth factor-10.
  Emoto H, Tagashira S, Mattei MG, Yamasaki M, Hashimoto G, Katsumata T, Negoro T, Nakatsuka M, Birnbaum D, Coulier F, Itoh N. Emoto H, et al. J Biol Chem. 1997 Sep 12;272(37):23191-4. doi: 10.1074/jbc.272.37.23191. J Biol Chem. 1997. PMID: 9287324
• Human fibroblast growth factor-18 stimulates fibroblast cell proliferation and is mapped to chromosome 14p11.
  Hu MC, Wang YP, Qiu WR. Hu MC, et al. Oncogene. 1999 Apr 22;18(16):2635-42. doi: 10.1038/sj.onc.1202616. Oncogene. 1999. PMID: 10353607
• FGF signaling network in the gastrointestinal tract (review).
  Katoh M, Katoh M. Katoh M, et al. Int J Oncol. 2006 Jul;29(1):163-8. Int J Oncol. 2006. PMID: 16773196 Review.
• Heparan sulfate fibroblast growth factor receptor complex: structure-function relationships.
  McKeehan WL, Kan M. McKeehan WL, et al. Mol Reprod Dev. 1994 Sep;39(1):69-81; discusison 81-2. doi: 10.1002/mrd.1080390112. Mol Reprod Dev. 1994. PMID: 7999363 Review.

Cited by

• Overexpression of fibroblast growth factor 13 ameliorates amyloid-β-induced neuronal damage.
  Li RM, Xiao L, Zhang T, Ren D, Zhu H. Li RM, et al. Neural Regen Res. 2023 Jun;18(6):1347-1353. doi: 10.4103/1673-5374.357902. Neural Regen Res. 2023. PMID: 36453422 Free PMC article.
• Regulatory module involving FGF13, miR-504, and p53 regulates ribosomal biogenesis and supports cancer cell survival.
  Bublik DR, Bursać S, Sheffer M, Oršolić I, Shalit T, Tarcic O, Kotler E, Mouhadeb O, Hoffman Y, Fuchs G, Levin Y, Volarević S, Oren M. Bublik DR, et al. Proc Natl Acad Sci U S A. 2017 Jan 24;114(4):E496-E505. doi: 10.1073/pnas.1614876114. Epub 2016 Dec 19. Proc Natl Acad Sci U S A. 2017. PMID: 27994142 Free PMC article.
• Fgf13 Identified as a Novel Cause of GEFS.
  Wong JC, Escayg A. Wong JC, et al. Epilepsy Curr. 2016 Mar-Apr;16(2):112-3. doi: 10.5698/1535-7511-16.2.112. Epilepsy Curr. 2016. PMID: 27073347 Free PMC article. No abstract available.
• Identification of genomic variants putatively targeted by selection during dog domestication.
  Cagan A, Blass T. Cagan A, et al. BMC Evol Biol. 2016 Jan 12;16:10. doi: 10.1186/s12862-015-0579-7. BMC Evol Biol. 2016. PMID: 26754411 Free PMC article.
• Parental binge alcohol abuse alters F1 generation hypothalamic gene expression in the absence of direct fetal alcohol exposure.
  Przybycien-Szymanska MM, Rao YS, Prins SA, Pak TR. Przybycien-Szymanska MM, et al. PLoS One. 2014 Feb 20;9(2):e89320. doi: 10.1371/journal.pone.0089320. eCollection 2014. PLoS One. 2014. PMID: 24586686 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Mouse Genome Informatics (MGI)

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal