Controlling gene expression in living cells through small molecule-RNA interactions - PubMed (original) (raw)
Controlling gene expression in living cells through small molecule-RNA interactions
G Werstuck et al. Science. 1998.
Abstract
Short RNA aptamers that specifically bind to a wide variety of ligands in vitro can be isolated from randomized pools of RNA. Here it is shown that small molecule aptamers also bound their ligand in vivo, enabling development of a method for controlling gene expression in living cells. Insertion of a small molecule aptamer into the 5' untranslated region of a messenger RNA allowed its translation to be repressible by ligand addition in vitro as well as in mammalian cells. The ability of small molecules to control expression of specific genes could facilitate studies in many areas of biology and medicine.
Similar articles
- RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities.
Wang Y, Killian J, Hamasaki K, Rando RR. Wang Y, et al. Biochemistry. 1996 Sep 24;35(38):12338-46. doi: 10.1021/bi960878w. Biochemistry. 1996. PMID: 8823168 - Minimal RNA constructs that specifically bind aminoglycoside antibiotics with high affinities.
Hamasaki K, Killian J, Cho J, Rando RR. Hamasaki K, et al. Biochemistry. 1998 Jan 13;37(2):656-63. doi: 10.1021/bi971095t. Biochemistry. 1998. PMID: 9425088 - Engineering a direct and inducible protein-RNA interaction to regulate RNA biology.
Belmont BJ, Niles JC. Belmont BJ, et al. ACS Chem Biol. 2010 Sep 17;5(9):851-61. doi: 10.1021/cb100070j. ACS Chem Biol. 2010. PMID: 20545348 - Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics.
Patel DJ, Suri AK. Patel DJ, et al. J Biotechnol. 2000 Mar;74(1):39-60. doi: 10.1016/s1389-0352(99)00003-3. J Biotechnol. 2000. PMID: 10943571 Review. - Chemical approaches to control gene expression.
Gottesfeld JM, Turner JM, Dervan PB. Gottesfeld JM, et al. Gene Expr. 2000;9(1-2):77-91. doi: 10.3727/000000001783992696. Gene Expr. 2000. PMID: 11097426 Free PMC article. Review.
Cited by
- Rational evolution for altering the ligand preference of estrogen receptor alpha.
Eerlings R, Gupta P, Lee XY, Nguyen T, El Kharraz S, Handle F, Smeets E, Moris L, Devlies W, Vandewinkel B, Thiry I, Ta DT, Gorkovskiy A, Voordeckers K, Henckaerts E, Pinheiro VB, Claessens F, Verstrepen KJ, Voet A, Helsen C. Eerlings R, et al. Protein Sci. 2024 Apr;33(4):e4940. doi: 10.1002/pro.4940. Protein Sci. 2024. PMID: 38511482 - Tapping the potential of synthetic riboswitches: reviewing the versatility of the tetracycline aptamer.
Kelvin D, Suess B. Kelvin D, et al. RNA Biol. 2023 Jan;20(1):457-468. doi: 10.1080/15476286.2023.2234732. RNA Biol. 2023. PMID: 37459466 Free PMC article. Review. - Biomimetic natural biomaterials for tissue engineering and regenerative medicine: new biosynthesis methods, recent advances, and emerging applications.
Liu S, Yu JM, Gan YC, Qiu XZ, Gao ZC, Wang H, Chen SX, Xiong Y, Liu GH, Lin SE, McCarthy A, John JV, Wei DX, Hou HH. Liu S, et al. Mil Med Res. 2023 Mar 28;10(1):16. doi: 10.1186/s40779-023-00448-w. Mil Med Res. 2023. PMID: 36978167 Free PMC article. Review. - Contemporary Progress and Opportunities in RNA-Targeted Drug Discovery.
Garner AL. Garner AL. ACS Med Chem Lett. 2023 Feb 23;14(3):251-259. doi: 10.1021/acsmedchemlett.3c00020. eCollection 2023 Mar 9. ACS Med Chem Lett. 2023. PMID: 36923915 Free PMC article. Review. - Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers.
Climent-Catala A, Ouldridge TE, Stan GV, Bae W. Climent-Catala A, et al. ACS Synth Biol. 2022 Feb 18;11(2):562-569. doi: 10.1021/acssynbio.1c00580. Epub 2022 Feb 8. ACS Synth Biol. 2022. PMID: 35133150 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical