The Bloom's syndrome helicase unwinds G4 DNA - PubMed (original) (raw)
Comparative Study
. 1998 Oct 16;273(42):27587-92.
doi: 10.1074/jbc.273.42.27587.
Affiliations
- PMID: 9765292
- DOI: 10.1074/jbc.273.42.27587
Free article
Comparative Study
The Bloom's syndrome helicase unwinds G4 DNA
H Sun et al. J Biol Chem. 1998.
Free article
Abstract
BLM, the gene that is defective in Bloom's syndrome, encodes a protein homologous to RecQ subfamily helicases that functions as a 3'-5' DNA helicase in vitro. We now report that the BLM helicase can unwind G4 DNA. The BLM G4 DNA unwinding activity is ATP-dependent and requires a short 3' region of single-stranded DNA. Strikingly, G4 DNA is a preferred substrate of the BLM helicase, as measured both by efficiency of unwinding and by competition. These results suggest that G4 DNA may be a natural substrate of BLM in vivo and that the failure to unwind G4 DNA may cause the genomic instability and increased frequency of sister chromatid exchange characteristic of Bloom's syndrome.
Similar articles
- Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity.
Brosh RM Jr, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA. Brosh RM Jr, et al. J Biol Chem. 2000 Aug 4;275(31):23500-8. doi: 10.1074/jbc.M001557200. J Biol Chem. 2000. PMID: 10825162 - Roles of the Bloom's syndrome helicase in the maintenance of genome stability.
Cheok CF, Bachrati CZ, Chan KL, Ralf C, Wu L, Hickson ID. Cheok CF, et al. Biochem Soc Trans. 2005 Dec;33(Pt 6):1456-9. doi: 10.1042/BST0331456. Biochem Soc Trans. 2005. PMID: 16246145 Review. - Novel pro- and anti-recombination activities of the Bloom's syndrome helicase.
Bugreev DV, Yu X, Egelman EH, Mazin AV. Bugreev DV, et al. Genes Dev. 2007 Dec 1;21(23):3085-94. doi: 10.1101/gad.1609007. Epub 2007 Nov 14. Genes Dev. 2007. PMID: 18003860 Free PMC article. - Competition between the DNA unwinding and strand pairing activities of the Werner and Bloom syndrome proteins.
Machwe A, Lozada EM, Xiao L, Orren DK. Machwe A, et al. BMC Mol Biol. 2006 Jan 13;7:1. doi: 10.1186/1471-2199-7-1. BMC Mol Biol. 2006. PMID: 16412221 Free PMC article. - Role of the BLM helicase in replication fork management.
Wu L. Wu L. DNA Repair (Amst). 2007 Jul 1;6(7):936-44. doi: 10.1016/j.dnarep.2007.02.007. Epub 2007 Mar 23. DNA Repair (Amst). 2007. PMID: 17363339 Review.
Cited by
- The G4 genome.
Maizels N, Gray LT. Maizels N, et al. PLoS Genet. 2013 Apr;9(4):e1003468. doi: 10.1371/journal.pgen.1003468. Epub 2013 Apr 18. PLoS Genet. 2013. PMID: 23637633 Free PMC article. - Small-molecule-induced DNA damage identifies alternative DNA structures in human genes.
Rodriguez R, Miller KM, Forment JV, Bradshaw CR, Nikan M, Britton S, Oelschlaegel T, Xhemalce B, Balasubramanian S, Jackson SP. Rodriguez R, et al. Nat Chem Biol. 2012 Feb 5;8(3):301-10. doi: 10.1038/nchembio.780. Nat Chem Biol. 2012. PMID: 22306580 Free PMC article. - Roles of DNA helicases in the mediation and regulation of homologous recombination.
Daley JM, Niu H, Sung P. Daley JM, et al. Adv Exp Med Biol. 2013;767:185-202. doi: 10.1007/978-1-4614-5037-5_9. Adv Exp Med Biol. 2013. PMID: 23161012 Free PMC article. - BLM unfolds G-quadruplexes in different structural environments through different mechanisms.
Wu WQ, Hou XM, Li M, Dou SX, Xi XG. Wu WQ, et al. Nucleic Acids Res. 2015 May 19;43(9):4614-26. doi: 10.1093/nar/gkv361. Epub 2015 Apr 20. Nucleic Acids Res. 2015. PMID: 25897130 Free PMC article. - G-quadruplexes are a source of vulnerability in BRCA2 deficient granule cell progenitors and medulloblastoma.
Keahi DL, Sanders MA, Paul MR, Webster ALH, Fang Y, Wiley TF, Shalaby S, Carroll TS, Chandrasekharappa SC, Sandoval-Garcia C, MacMillan ML, Wagner JE, Hatten ME, Smogorzewska A. Keahi DL, et al. bioRxiv [Preprint]. 2024 Jul 22:2024.07.20.604431. doi: 10.1101/2024.07.20.604431. bioRxiv. 2024. PMID: 39091814 Free PMC article. Preprint.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources