Determination of 16S rRNA sequences of enterococci and application to species identification of nonmotile Enterococcus gallinarum isolates - PubMed (original) (raw)

Comparative Study

Determination of 16S rRNA sequences of enterococci and application to species identification of nonmotile Enterococcus gallinarum isolates

R Patel et al. J Clin Microbiol. 1998 Nov.

Abstract

The 16S rRNA sequences of enterococcal species E. faecium, E. faecalis, E. gallinarum, E. casseliflavus/flavescens, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius are presented herein. These data were utilized to confirm the species identification of two nonmotile E. gallinarum isolates which had been previously phenotypically identified as E. faecium. The implications of this finding are discussed.

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Figures

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 1

FIG. 1

16S rRNA sequences of E. faecium, E. faecalis, E. gallinarum, the E. casseliflavus/flavescens group, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius. Position 99 corresponds to position 100 of the Escherichia coli 16S rRNA gene. R = A or G; Y = C or T.

FIG. 2

FIG. 2

Distance matrix tree of Enterococcus spp. derived from sequence homology determinations of 16S rRNA. The tree was constructed by the neighbor-joining method (23). The tree was rooted by using E. seriolicida as an outgroup. The scale bar represents a 1% difference in nucleotide sequence, as determined by taking the sum of all of the horizontal lines connecting two species.

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