Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control - PubMed (original) (raw)
Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control
E Frahm et al. Syst Appl Microbiol. 1998 Aug.
Abstract
Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.
Similar articles
- Rapid parallel detection of hygienically relevant microorganisms in water samples by PCR and specific hybridization in microtiter plates.
Frahm E, Heiber I, Ludwig W, Obst U. Frahm E, et al. Syst Appl Microbiol. 2001 Nov;24(3):423-9. doi: 10.1078/0723-2020-00056. Syst Appl Microbiol. 2001. PMID: 11822680 - Oligonucleotide microarray for identification of Enterococcus species.
Lehner A, Loy A, Behr T, Gaenge H, Ludwig W, Wagner M, Schleifer KH. Lehner A, et al. FEMS Microbiol Lett. 2005 May 1;246(1):133-42. doi: 10.1016/j.femsle.2005.04.002. FEMS Microbiol Lett. 2005. PMID: 15869972 - Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp. 2. Pheno- and genotypic criteria.
Domig KJ, Mayer HK, Kneifel W. Domig KJ, et al. Int J Food Microbiol. 2003 Dec 1;88(2-3):165-88. doi: 10.1016/s0168-1605(03)00178-8. Int J Food Microbiol. 2003. PMID: 14596988 Review. - Taxonomical changes in intestinal (faecal) enterococci and streptococci: consequences on their use as indicators of faecal contamination in drinking water.
Leclerc H, Devriese LA, Mossel DA. Leclerc H, et al. J Appl Bacteriol. 1996 Nov;81(5):459-66. doi: 10.1111/j.1365-2672.1996.tb03533.x. J Appl Bacteriol. 1996. PMID: 8939023 Review. No abstract available.
Cited by
- Spatial organisation of microbiota in quiescent adenoiditis and tonsillitis.
Swidsinski A, Göktas O, Bessler C, Loening-Baucke V, Hale LP, Andree H, Weizenegger M, Hölzl M, Scherer H, Lochs H. Swidsinski A, et al. J Clin Pathol. 2007 Mar;60(3):253-60. doi: 10.1136/jcp.2006.037309. Epub 2006 May 12. J Clin Pathol. 2007. PMID: 16698947 Free PMC article. - Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.
Peters RP, van Agtmael MA, Simoons-Smit AM, Danner SA, Vandenbroucke-Grauls CM, Savelkoul PH. Peters RP, et al. J Clin Microbiol. 2006 Nov;44(11):4186-8. doi: 10.1128/JCM.01085-06. J Clin Microbiol. 2006. PMID: 17088371 Free PMC article. - Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease.
Swidsinski A, Weber J, Loening-Baucke V, Hale LP, Lochs H. Swidsinski A, et al. J Clin Microbiol. 2005 Jul;43(7):3380-9. doi: 10.1128/JCM.43.7.3380-3389.2005. J Clin Microbiol. 2005. PMID: 16000463 Free PMC article. - Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences.
Mitterer G, Huber M, Leidinger E, Kirisits C, Lubitz W, Mueller MW, Schmidt WM. Mitterer G, et al. J Clin Microbiol. 2004 Mar;42(3):1048-57. doi: 10.1128/JCM.42.3.1048-1057.2004. J Clin Microbiol. 2004. PMID: 15004052 Free PMC article. - Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species.
Tsiodras S, Gold HS, Coakley EP, Wennersten C, Moellering RC Jr, Eliopoulos GM. Tsiodras S, et al. J Clin Microbiol. 2000 Nov;38(11):3991-3. doi: 10.1128/JCM.38.11.3991-3993.2000. J Clin Microbiol. 2000. PMID: 11060057 Free PMC article.