Identification and expression of two novel CLIP-170/Restin isoforms expressed predominantly in muscle - PubMed (original) (raw)
Identification and expression of two novel CLIP-170/Restin isoforms expressed predominantly in muscle
L Griparic et al. Biochim Biophys Acta. 1998.
Free article
Abstract
CLIP-170 and Restin, microtubule-binding proteins originally cloned from human cells, are identical except for a stretch of 35 amino acids present in Restin, but missing from CLIP-170. Here we present the discovery of two novel isoforms of the CLIP-170/Restin gene in both chickens and humans. One of the new isoforms, named CLIP-170(11), contains an 11 amino acid insert instead of the 35 amino acid insert found in Restin. Eight of these 11 amino acids, including a helix-breaking proline residue, are perfectly conserved between chickens and humans. The second new isoform, named CLIP-170(11+35), contains both the 11 and 35 amino acid inserts in tandem. PCR analysis of chicken genomic DNA revealed that all four isoforms result from differential splicing of two exons in a region of the CLIP-170 gene that contains approximately 8.6 kb of intervening sequence. We found that the CLIP-170(11) and CLIP-170(11+35) are expressed preferentially in muscle tissues. Chicken and human skeletal muscle express predominantly CLIP-170(11) and to a lesser extent CLIP-170 and CLIP-170(11+35). Adult chicken cardiac and smooth muscles also express CLIP-170(11) and CLIP-170(11+35), but CLIP-170 is the predominant isoform in these muscles as it is in all other tissues except brain. The ratios of CLIP-170 isoform expression found in embryonic and adult chicken cardiac muscles reveal that isoform expression is regulated differentially in different developmental stages as well as in different tissues.
Similar articles
- Cloning and expression of chicken CLIP-170 and restin isoforms.
Griparic L, Volosky JM, Keller TC 3rd. Griparic L, et al. Gene. 1998 Jan 12;206(2):195-208. doi: 10.1016/s0378-1119(97)00585-4. Gene. 1998. PMID: 9469933 - Molecular cloning and functional characterization of chicken brain tau: isoforms with up to five tandem repeats.
Yoshida H, Goedert M. Yoshida H, et al. Biochemistry. 2002 Dec 24;41(51):15203-11. doi: 10.1021/bi026464m. Biochemistry. 2002. PMID: 12484758 - Differential usage of two 5' splice sites in a complex exon generates additional protein sequence complexity in chicken CLIP-170 isoforms.
Griparic L, Keller TC 3rd. Griparic L, et al. Biochim Biophys Acta. 1999 Mar 8;1449(2):119-24. doi: 10.1016/s0167-4889(99)00003-8. Biochim Biophys Acta. 1999. PMID: 10082970 - Developmental and muscle-specific regulation of avian fast skeletal troponin T isoform expression by mRNA splicing.
Bucher EA, de la Brousse FC, Emerson CP Jr. Bucher EA, et al. J Biol Chem. 1989 Jul 25;264(21):12482-91. J Biol Chem. 1989. PMID: 2745456 - The chicken vimentin gene: aspects of organization and transcription during myogenesis.
Zehner ZE, Paterson BM. Zehner ZE, et al. Ann N Y Acad Sci. 1985;455:79-94. doi: 10.1111/j.1749-6632.1985.tb50405.x. Ann N Y Acad Sci. 1985. PMID: 3909887 Review.
Cited by
- Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP.
Jakka P, Bhargavi B, Namani S, Murugan S, Splitter G, Radhakrishnan G. Jakka P, et al. J Immunol. 2018 Jan 15;200(2):704-714. doi: 10.4049/jimmunol.1601559. Epub 2017 Dec 8. J Immunol. 2018. PMID: 29222167 Free PMC article. - Biological functions of melanoma-associated antigens.
Xiao J, Chen HS. Xiao J, et al. World J Gastroenterol. 2004 Jul 1;10(13):1849-53. doi: 10.3748/wjg.v10.i13.1849. World J Gastroenterol. 2004. PMID: 15222021 Free PMC article. Review. - The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis.
Akhmanova A, Mausset-Bonnefont AL, van Cappellen W, Keijzer N, Hoogenraad CC, Stepanova T, Drabek K, van der Wees J, Mommaas M, Onderwater J, van der Meulen H, Tanenbaum ME, Medema RH, Hoogerbrugge J, Vreeburg J, Uringa EJ, Grootegoed JA, Grosveld F, Galjart N. Akhmanova A, et al. Genes Dev. 2005 Oct 15;19(20):2501-15. doi: 10.1101/gad.344505. Genes Dev. 2005. PMID: 16230537 Free PMC article.