Differentiation method-dependent expression of leptin in adipocyte cell lines - PubMed (original) (raw)
. 1998 Oct 9;251(1):225-9.
doi: 10.1006/bbrc.1998.9433.
Affiliations
- PMID: 9790935
- DOI: 10.1006/bbrc.1998.9433
Differentiation method-dependent expression of leptin in adipocyte cell lines
L J Slieker et al. Biochem Biophys Res Commun. 1998.
Abstract
Leptin, the product of the ob gene, is expressed exclusively in adipose tissue. However, adipocyte cell lines, such as 3T3-L1 adipocytes, have generally been reported to express extremely low levels of leptin mRNA. We compared 3T3-L1's to the closely related line 3T3-F442A, and to another murine adipocyte line, TA1. TA1 cells, when differentiated by indomethacin/insulin treatment, express leptin at levels greater than those of 3T3-L1 adipocytes differentiated by the traditional methylisobutylxanthine/dexamethasone/insulin protocol. However, when 3T3-L1's are differentiated in the presence of indomethacin/insulin their expression levels of leptin increase dramatically. 3T3-F442A preadipocytes also express high levels of leptin when differentiated in the presence of T3 and insulin, but when differentiated in the presence of indomethacin/insulin, expression levels drop precipitously. These changes in leptin mRNA and protein expression are not reflected by changes in CCAAT/enhancer binding protein-alpha (c/EBPalpha), peroxizomal proliferator activated receptor-gamma (PPARgamma), lipoprotein lipase (LPL), fatty-acid binding protein aP2 or uncoupling protein-2 (UCP2) mRNA levels, and suggest a mechanism unique to the leptin gene.
Copyright 1998 Academic Press.
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