The major resistance gene cluster in lettuce is highly duplicated and spans several megabases - PubMed (original) (raw)
Comparative Study
The major resistance gene cluster in lettuce is highly duplicated and spans several megabases
B C Meyers et al. Plant Cell. 1998 Nov.
Abstract
At least 10 Dm genes conferring resistance to the oomycete downy mildew fungus Bremia lactucae map to the major resistance cluster in lettuce. We investigated the structure of this cluster in the lettuce cultivar Diana, which contains Dm3. A deletion breakpoint map of the chromosomal region flanking Dm3 was saturated with a variety of molecular markers. Several of these markers are components of a family of resistance gene candidates (RGC2) that encode a nucleotide binding site and a leucine-rich repeat region. These motifs are characteristic of plant disease resistance genes. Bacterial artificial chromosome clones were identified by using duplicated restriction fragment length polymorphism markers from the region, including the nucleotide binding site-encoding region of RGC2. Twenty-two distinct members of the RGC2 family were characterized from the bacterial artificial chromosomes; at least two additional family members exist. The RGC2 family is highly divergent; the nucleotide identity was as low as 53% between the most distantly related copies. These RGC2 genes span at least 3.5 Mb. Eighteen members were mapped on the deletion breakpoint map. A comparison between the phylogenetic and physical relationships of these sequences demonstrated that closely related copies are physically separated from one another and indicated that complex rearrangements have shaped this region. Analysis of low-copy genomic sequences detected no genes, including RGC2, in the Dm3 region, other than sequences related to retrotransposons and transposable elements. The related but divergent family of RGC2 genes may act as a resource for the generation of new resistance phenotypes through infrequent recombination or unequal crossing over.
Similar articles
- Receptor-like genes in the major resistance locus of lettuce are subject to divergent selection.
Meyers BC, Shen KA, Rohani P, Gaut BS, Michelmore RW. Meyers BC, et al. Plant Cell. 1998 Nov;10(11):1833-46. doi: 10.1105/tpc.10.11.1833. Plant Cell. 1998. PMID: 9811792 Free PMC article. - Molecular analysis of irradiation-induced and spontaneous deletion mutants at a disease resistance locus in Lactuca sativa.
Anderson PA, Okubara PA, Arroyo-Garcia R, Meyers BC, Michelmore RW. Anderson PA, et al. Mol Gen Genet. 1996 Jun 12;251(3):316-25. doi: 10.1007/BF02172522. Mol Gen Genet. 1996. PMID: 8676874 - Multiple genetic processes result in heterogeneous rates of evolution within the major cluster disease resistance genes in lettuce.
Kuang H, Woo SS, Meyers BC, Nevo E, Michelmore RW. Kuang H, et al. Plant Cell. 2004 Nov;16(11):2870-94. doi: 10.1105/tpc.104.025502. Epub 2004 Oct 19. Plant Cell. 2004. PMID: 15494555 Free PMC article. - Silencing of the major family of NBS-LRR-encoding genes in lettuce results in the loss of multiple resistance specificities.
Wroblewski T, Piskurewicz U, Tomczak A, Ochoa O, Michelmore RW. Wroblewski T, et al. Plant J. 2007 Sep;51(5):803-18. doi: 10.1111/j.1365-313X.2007.03182.x. Epub 2007 Jun 22. Plant J. 2007. PMID: 17587302 - The disease resistance gene Dm3 is infrequent in natural populations of Lactuca serriola due to deletions and frequent gene conversions at the RGC2 locus.
Kuang H, Ochoa OE, Nevo E, Michelmore RW. Kuang H, et al. Plant J. 2006 Jul;47(1):38-48. doi: 10.1111/j.1365-313X.2006.02755.x. Epub 2006 Jun 7. Plant J. 2006. PMID: 16762035
Cited by
- Identification and characterization of resistance quantitative trait loci against bacterial wilt caused by the Ralstonia solanacearum species complex in potato.
Habe I, Miyatake K. Habe I, et al. Mol Breed. 2022 Aug 23;42(9):50. doi: 10.1007/s11032-022-01321-9. eCollection 2022 Sep. Mol Breed. 2022. PMID: 37313419 Free PMC article. - Partially Resistant Avocado Rootstock Dusa® Shows Prolonged Upregulation of Nucleotide Binding-Leucine Rich Repeat Genes in Response to Phytophthora cinnamomi Infection.
Fick A, Swart V, Backer R, Bombarely A, Engelbrecht J, van den Berg N. Fick A, et al. Front Plant Sci. 2022 Mar 11;13:793644. doi: 10.3389/fpls.2022.793644. eCollection 2022. Front Plant Sci. 2022. PMID: 35360305 Free PMC article. - Mapping and characterization of the Rx3 gene for resistance to Xanthomonas euvesicatoria pv. euvesicatoria race T1 in tomato.
Meng G, Xiao Y, Li A, Qian Z, Xie Y, Yang L, Lin H, Yang W. Meng G, et al. Theor Appl Genet. 2022 May;135(5):1637-1656. doi: 10.1007/s00122-022-04059-2. Epub 2022 Feb 25. Theor Appl Genet. 2022. PMID: 35217878 - Reciprocal adaptation of rice and Xanthomonas oryzae pv. oryzae: cross-species 2D GWAS reveals the underlying genetics.
Zhang F, Hu Z, Wu Z, Lu J, Shi Y, Xu J, Wang X, Wang J, Zhang F, Wang M, Shi X, Cui Y, Vera Cruz C, Zhuo D, Hu D, Li M, Wang W, Zhao X, Zheng T, Fu B, Ali J, Zhou Y, Li Z. Zhang F, et al. Plant Cell. 2021 Aug 31;33(8):2538-2561. doi: 10.1093/plcell/koab146. Epub 2021 Jun 2. Plant Cell. 2021. PMID: 34467412 Free PMC article. - Tightly linked Rps12 and Rps13 genes provide broad-spectrum Phytophthora resistance in soybean.
Sahoo DK, Das A, Huang X, Cianzio S, Bhattacharyya MK. Sahoo DK, et al. Sci Rep. 2021 Aug 19;11(1):16907. doi: 10.1038/s41598-021-96425-1. Sci Rep. 2021. PMID: 34413429 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11751-6 - PubMed
- Genetics. 1993 Jan;133(1):119-25 - PubMed
- Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828-32 - PubMed
- Nucleic Acids Res. 1995 Nov 11;23(21):4407-14 - PubMed
- Genetics. 1994 Jul;137(3):867-74 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous