Deformation-induced ATP release from red blood cells requires CFTR activity - PubMed (original) (raw)
Deformation-induced ATP release from red blood cells requires CFTR activity
R S Sprague et al. Am J Physiol. 1998 Nov.
Abstract
Recently, it was reported that rabbit and human red blood cells (RBCs) release ATP in response to mechanical deformation. Here we investigate the hypothesis that the activity of the cystic fibrosis transmembrane conductance regulator (CFTR), a member of the ATP binding cassette, is required for deformation-induced ATP release from RBCs. Incubation of rabbit RBCs with either of two inhibitors of CFTR activity, glibenclamide (10 microM) or niflumic acid (20 microM), resulted in inhibition of deformation-induced ATP release. To demonstrate the contribution of CFTR to deformation-induced ATP release from human RBCs, cells from healthy humans, patients with cystic fibrosis (CF), or patients with chronic obstructive lung disease (COPD) unrelated to CF were studied. RBCs of healthy humans and COPD patients released ATP in response to mechanical deformation. In contrast, deformation of RBCs from patients with CF did not result in ATP release. We conclude that deformation-induced ATP release from rabbit and human RBCs requires CFTR activity, suggesting a previously unrecognized role for CFTR in the regulation of vascular resistance.
Similar articles
- Participation of cAMP in a signal-transduction pathway relating erythrocyte deformation to ATP release.
Sprague RS, Ellsworth ML, Stephenson AH, Lonigro AJ. Sprague RS, et al. Am J Physiol Cell Physiol. 2001 Oct;281(4):C1158-64. doi: 10.1152/ajpcell.2001.281.4.C1158. Am J Physiol Cell Physiol. 2001. PMID: 11546651 - Impaired release of ATP from red blood cells of humans with primary pulmonary hypertension.
Sprague RS, Stephenson AH, Ellsworth ML, Keller C, Lonigro AJ. Sprague RS, et al. Exp Biol Med (Maywood). 2001 May;226(5):434-9. doi: 10.1177/153537020122600507. Exp Biol Med (Maywood). 2001. PMID: 11393171 - Erythrocytes of humans with cystic fibrosis fail to stimulate nitric oxide synthesis in isolated rabbit lungs.
Liang G, Stephenson AH, Lonigro AJ, Sprague RS. Liang G, et al. Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1580-5. doi: 10.1152/ajpheart.00807.2004. Epub 2004 Dec 9. Am J Physiol Heart Circ Physiol. 2005. PMID: 15591098 - [The mechanism of ATP release as an autocrine/paracrine molecule].
Katsuragi T, Migita K. Katsuragi T, et al. Nihon Yakurigaku Zasshi. 2004 Jun;123(6):382-8. doi: 10.1254/fpj.123.382. Nihon Yakurigaku Zasshi. 2004. PMID: 15170077 Review. Japanese. - Exploiting species differences to understand the CFTR Cl- channel.
Bose SJ, Scott-Ward TS, Cai Z, Sheppard DN. Bose SJ, et al. Biochem Soc Trans. 2015 Oct;43(5):975-82. doi: 10.1042/BST20150129. Biochem Soc Trans. 2015. PMID: 26517912 Review.
Cited by
- Mechanical effects of muscle contraction increase intravascular ATP draining quiescent and active skeletal muscle in humans.
Crecelius AR, Kirby BS, Richards JC, Dinenno FA. Crecelius AR, et al. J Appl Physiol (1985). 2013 Apr;114(8):1085-93. doi: 10.1152/japplphysiol.01465.2012. Epub 2013 Feb 21. J Appl Physiol (1985). 2013. PMID: 23429876 Free PMC article. - Hydroxyurea stimulates the release of ATP from rabbit erythrocytes through an increase in calcium and nitric oxide production.
Raththagala M, Karunarathne W, Kryziniak M, McCracken J, Spence DM. Raththagala M, et al. Eur J Pharmacol. 2010 Oct 25;645(1-3):32-8. doi: 10.1016/j.ejphar.2010.07.012. Epub 2010 Jul 23. Eur J Pharmacol. 2010. PMID: 20655902 Free PMC article. - Stimulation of rat erythrocyte P2X7 receptor induces the release of epoxyeicosatrienoic acids.
Jiang H, Zhu AG, Mamczur M, Falck JR, Lerea KM, McGiff JC. Jiang H, et al. Br J Pharmacol. 2007 Aug;151(7):1033-40. doi: 10.1038/sj.bjp.0707311. Epub 2007 Jun 11. Br J Pharmacol. 2007. PMID: 17558440 Free PMC article. - Physiological regulation of ATP release at the apical surface of human airway epithelia.
Okada SF, Nicholas RA, Kreda SM, Lazarowski ER, Boucher RC. Okada SF, et al. J Biol Chem. 2006 Aug 11;281(32):22992-3002. doi: 10.1074/jbc.M603019200. Epub 2006 Jun 5. J Biol Chem. 2006. PMID: 16754672 Free PMC article. - Swelling-induced, CFTR-independent ATP release from a human epithelial cell line: lack of correlation with volume-sensitive cl(-) channels.
Hazama A, Shimizu T, Ando-Akatsuka Y, Hayashi S, Tanaka S, Maeno E, Okada Y. Hazama A, et al. J Gen Physiol. 1999 Oct;114(4):525-33. doi: 10.1085/jgp.114.4.525. J Gen Physiol. 1999. PMID: 10498671 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources